武汉云克隆科技股份有限公司

Abstract：Neprilysin is a zinc-dependent endopeptidase. It is ubiquitous in distribution and promiscuous in function, with >50 putative peptide substrates with varying levels of in vitro and/or in vivo evidence of functional relevance. In the first part of this review, we discuss the genetic, structural, substrate, and pathophysiological aspects of neprilysin. We incorporate information provided by genetically modified models, as well as pre-clinical and clinical data from investigations of synthetic neprilysin inhibitors. We next highlight the value of neprilysin as a biotarget and weigh the clinical benefits of synthetic neprilysin inhibitors, either alone or in combination with antagonists of the renin–angiotensin system. Finally, we provide evidence about soluble neprilysin as a biomarker surrogate in patients with heart failure and identify important gaps that require further research before soluble neprilysin is used clinically. In sum, neprilysin is a versatile, veteran player returning yet again to center stage after an eventful career spanning >40 years.

摘要：脑啡肽酶是锌依赖性内肽酶。它在功能上分布普遍且混杂，具有> 50个假定肽底物，具有不同水平的体外和/或体内功能相关性证据。在本综述的第一部分，我们讨论脑啡肽酶的遗传，结构，底物和病理生理学方面。我们整合了基因修饰模型提供的信息，以及来自合成脑啡肽酶抑制剂调查的临床前和临床数据。我们接下来强调了脑啡肽酶作为生物靶点的价值，并单独或与肾素 - 血管紧张素系统的拮抗剂组合，衡量合成脑啡肽酶抑制剂的临床益处。最后，我们提供了关于可溶性脑啡肽酶作为心力衰竭患者生物标志物替代物的证据，并确定了在临床使用可溶性脑啡肽酶之前需要进一步研究的重要空白。总之，脑啡肽酶一种多功能物质，在经历了超过40年的研究后，又一次成为的研究的热点。

使用试剂原文信息：Thirty serum samples were processed in duplicate, each from a different aliquot stored at –80C. The 4 commercial assays used were sNEP Aviscera (Aviscera Bioscience, Santa Clara, California), sNEP USCN (USCN Life Science, Wuhan, China), sNEP FIVEphoton (FIVEphoton Biochemicals, San Diego, California), and sNEP Boster (BosterBio, Pleasanton, California). An alarming lack of correlation was observed between assays. sNEP ¼ soluble neprilysin.

Morover, Goliash et al. and Vodovar et al. used the USCN Life Science (Wuhan, China)kit, which provides the closest relationship with Aviscera Bioscience.