调节宿主巨噬细胞的表型以增强充满MSC的水凝胶中的骨生成:设计葡甘露聚糖涂层材料

文献 Modulating the phenotype of host macrophages to enhance osteogenesis in MSC-laden hydrogels: Design of a glucomannan coating material. 发表在 BIOMATERIALS 原文链接

Abstract: The biomaterials-host interaction is a dynamic process in which macrophages play a vital role of regulation. Depending on the biochemical signals they sense, these highly plastic cells can mediate the immune response against the implanted scaffolds and/or exert regenerative potency to varying extent. Designing appropriate 'exterior signals' for scaffolds may exploit the power of endogenous macrophages to aid the regeneration of engineered tissues. To realise this goal, this study devised an injectable, instantaneously-solidifying coating material (acBSP) based on a unique, macrophage-affinitive glucomannan polysaccharide. Coating of three-dimensional hydrogel constructs with acBSP was rapid, neat and complete, requiring neither chemical reactions nor harsh conditions. Comprehensive in vitro analyses indicated that acBSP efficiently facilitated the adhesion and activation of macrophages and notably induced the macrophages to express pro-osteogenic/-angiogenic genes. Further in vivo assessment of acBSP-coated, mesenchymal stem cells-laden hydrogels in a murine dorsal subcutaneous pocket model demonstrated efficient macrophage activation, desirable scaffold-tissue integration and improved osteogenic differentiation in the delivered cells. In summary, by activating macrophages into a pro-osteogenic phenotype, the acBSP coating has demonstrated its competency as an innovative, open and efficacious platform to harness the power of host immunity for enhancing the regenerative performance of engineered tissue constructs.


摘要:生物材料与宿主的相互作用是巨噬细胞发挥重要调节作用的动态过程。根据它们感知的生化信号,这些高度塑性的细胞可以介导针对植入的支架的免疫应答和/或在不同程度上施加再生效力。为脚手架设计合适的“外部信号”可以利用内源性巨噬细胞的力量来帮助工程组织的再生。为了实现这一目标,本研究设计了一种基于独特的巨噬细胞亲和性葡甘露聚糖多糖的可注射瞬间固化涂层材料(acBSP)。用acBSP涂覆三维水凝胶构建体是快速,整齐和完整的,既不需要化学反应也不需要苛刻的条件。全面的体外分析表明,acBSP有效地促进了巨噬细胞的粘附和活化,并且显着诱导巨噬细胞表达促成骨原/血管生成基因。在鼠背部皮下袋模型中进一步体内评估acBSP包被的,间充质干细胞负载的水凝胶显示有效的巨噬细胞激活,期望的支架 - 组织整合和改善的递送细胞中的成骨分化。总之,通过激活巨噬细胞成为促成骨表型,acBSP涂层已经证明了其作为利用宿主免疫力来增强工程化组织构建体的再生性能的创新,开放和有效平台的能力。


使用试剂原文信息:For this assay, we respectively assessed the OSM and OCN levels in conditioned media and the homogenate of implanted scaffolds using ELISA assay kits (Cloud-Clone Corp.) in line with the manufacturers’ instructions. Each group contained three replicates.



文献相关产品 SEA110HuCEC673Hu 由云克隆研发生产