武汉云克隆科技股份有限公司

Abstract: Incorporation of bioactive drugs and biofunctionalization of polyester fibrous scaffolds are essential means to improve their bio-functions and histocompatibility for regenerative medicine. However, it is still a challenge to biofunctionalize such drug carriers via traditional biochemical methods while maintaining their properties without changes in drug activity and loading ratio. Here, we demonstrated a facile approach for biofunctionalization of PLGA fibrous scaffolds with various molecules (i.e., PEG polymer, RGD peptide and bFGF growth factor for cell repellent, adhesion and proliferation, respectively) via mussel-Inspired poly(dopamine) (PDA) coating in aqueous solution. By virtue of the mild and efficient nature of this approach, the drug-loaded PLGA fibers could be easily biofunctionalized and showed negligible effects on the scaffold properties, especially drug activity and loading ratio. Further, in vivo study showed that, a ginsenoside-Rg3-loaded fibrous scaffold functionalized with bFGF growth factor could not only promote the early-stage wound healing in rabbit ear wounds (bio-signal from bFGF), but also inhibit later-stage hypertrophic scars formation (release of Rg3 drug). Therefore, the mussel-inspired method for bio-modification provides a facile and effective strategy to combine drug and bio-function in one system, thus facilitating a synergistic effect of drug-therapy and bio-signal when such biomaterial is used for regenerative medicine.

摘要：生物活性药物结合聚酯纤维支架的生物功能化是改善其再生医学的生物功能和组织相容性的重要手段。然而，通过传统的生物化学方法对这些药物载体进行生物功能化，同时保持它们的性质而不改变药物活性和加载比例仍然是一个挑战。在这里，我们展示了通过贻贝激发多巴胺（PDA）涂层将PLGA纤维支架与各种分子（即PEG聚合物，RGD肽和bFGF生长因子分别用于细胞排斥，粘附和增殖）生物功能化的简便方法在水溶液中。由于这种方法的温和而有效的性质，载药PLGA纤维可以很容易生物功能化，并且对支架性质，特别是药物活性和负载比率的影响可以忽略不计。此外，体内研究表明，用bFGF生长因子功能化的载有人参皂苷-Rg3的纤维支架不仅可以促进兔耳创伤（来自bFGF的生物信号）的早期伤口愈合，而且还抑制后期肥大疤痕形成（释放Rg3药物）。因此，采用贻贝衍生的生物修饰方法提供了一种简单而有效的策略，将药物和生物功能结合在一个系统中，从而促进了这种生物材料用于再生医学时的药物治疗和生物信号的协同效应。

使用试剂原文信息：ELISA (Uscn Life Science Inc., Wuhan, China) was used to determine the amount of unbound bFGF in the wash buffer according to manufacturer's instructions