武汉云克隆科技股份有限公司

Abstract: Surfactant derived protein B (SPB) and plasma receptor for advanced glycation end products (RAGE) have been proposed as markers of lung injury. The former is produced specifically by pneumocytes while RAGE production is present in several body tissues. Cardiopulmonary bypass (CPB) generates a transient lung injury. We measured SPB and RAGE in plasma before surgery and after CPB, as well as 24 h and 48 h later. We analysed plasma samples from 20 subjects scheduled for elective coronary artery bypass grafting. We performed a quantitative analysis of plasma levels of RAGE and SPB mature form (8 kDa) by ELISA and a semi-quantitative analysis of SPB immature form (~ 40 kDa) by Western blotting. Surgery procedures were uneventful. After CPB RAGE median (75th-25th interquartile difference) increased from 633 (539) pg·mL⁻¹ to 1,362 (557) pg·mL⁻¹ (p < 0.01), while mature SPB increased from 5,587 (3,089) ng·mL⁻¹ to 20,307 (19,873) ng·mL⁻¹ (p < 0.01). RAGE and mature SPB returned to normal values within 48 h. This behaviour was confirmed when RAGE and SPB were normalised for protein content. Parallel changes were observed for immature SPB. Plasma RAGE and SPBs are sensitive and rapid markers of lung distress.

摘要：已提出表面活性剂衍生蛋白B（SPB）和晚期糖基化终产物（RAGE）的血浆受体作为肺损伤的标志物。前者是由肺细胞特异性产生的，而RAGE的产生则存在于几种身体组织中。体外循环（CPB）会产生一过性肺损伤。我们在手术前和CPB后以及24小时和48小时后测量血浆中的SPB和RAGE。我们分析了来自计划用于选择性冠状动脉搭桥术的20位受试者的血浆样品。我们通过ELISA和通过蛋白质印迹法对SPB未成熟形式（〜40kDa）的半定量分析进行RAGE和SPB成熟形式（8kDa）的血浆水平的定量分析。手术过程很顺利。在CPB RAGE中值（第75位至第25位具有四倍差异）从633（539）pg·mL^-1增加至1362（557）pg·mL^-1（p <0.01）后，成熟SPB从5,587（3,089）ng·mL -1到20,307（19,873）ng·mL -1（p <0.01）。48小时内RAGE和成熟SPB恢复正常值。当RAGE和SPB对蛋白质含量进行归一化时，这种行为得到了证实。对未成熟的SPB观察到平行变化。血浆RAGE和SPBs是肺部窘迫的敏感和快速标记。

使用试剂原文信息：The quantitative analysis of the levels of the mature form (8 kDa) of SPB was performed by an ELISA purchased from Uscn Life Science Inc. (Wuhan, China). Briefly, the microtitre plate provided in this kit was pre-coated with an antibody specific to the mature SPB.