α1-抗胰蛋白酶(a1AT)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Alpha-1-Antitrypsin (a1AT)
SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1
- 编号KSB697Ra01
- 物种Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围7.81-500ng/mL
- 可适灵敏度3.5ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7798¥ 12996¥ 23393¥ 45486¥ 77976
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
特异性
试剂盒中的抗体对α1-抗胰蛋白酶(a1AT)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB697Ra01 | α1-抗胰蛋白酶(a1AT)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB697Ra01 | α1-抗胰蛋白酶(a1AT)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB697Ra71 | α1-抗胰蛋白酶(a1AT)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB697Ra21 | α1-抗胰蛋白酶(a1AT)单克隆抗体 | WB. |
| MEB697Ra | α1-抗胰蛋白酶(a1AT)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEB697Ra | α1-抗胰蛋白酶(a1AT)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB697Ra | α1-抗胰蛋白酶(a1AT)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB697Ra01 | α1-抗胰蛋白酶(a1AT)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Journal of Biotechnology | Expression and characterization of recombinant human alpha-antitrypsin in transgenic rice seed[PubMed: 23376844] |
| Sci Rep | Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis[PubMed: 26634430] |
| PLoS One | Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study[PubMed: 25955845] |
| Respir Care | Diagnostic Utility of Biomarkers in COPD[PubMed: 26106205] |
| Acta Anaesthesiologica Scandinavica | Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach[Pubmed:29315472] |
| Journal of Clinical Medicine | Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients[Pubmed: 30486327] |
| Journal of Proteomics | Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection[Pubmed: 31706945] |
| Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J[Pubmed: 32585848] |
| Pediatrics International | Prenatal diagnosis of maternal serum from mothers carrying β‐thalassemic fetus[34559910] |