血管紧张素原(AGT)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Angiotensinogen (AGT)
ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)
- 编号KSA797Hu01
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围3.12-200ng/mL
- 可适灵敏度1.31ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 6804¥ 11340¥ 20412¥ 39690¥ 68040
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对血管紧张素原(AGT)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA797Hu02 | 血管紧张素原(AGT)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA797Hu01 | 血管紧张素原(AGT)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA797Hu61 | 血管紧张素原(AGT)活性蛋白 | Cell culture; Activity Assays. |
| EPA797Hu61 | 血管紧张素原(AGT)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA797Hu01 | 血管紧张素原(AGT)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA797Hu02 | 血管紧张素原(AGT)多克隆抗体 | WB,IHC,ICC/IF |
| MAA797Hu22 | 血管紧张素原(AGT)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA797Hu | 血管紧张素原(AGT)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA797Hu | 血管紧张素原(AGT)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA797Hu | 血管紧张素原(AGT)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA797Hu | 血管紧张素原(AGT)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA797Hu01 | 血管紧张素原(AGT)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Transplant International | Elevated urinary angiotensinogen a marker of intrarenal renin angiotensin system in hypertensive renal transplant recipients: does it play a role in development of proteinuria in hypertensive renal transplant patients?[Wiley: source] |
| Amyloid | The relation between urinary angiotensinogen and proteinuria in renal AA amyloidosis patients[PubMed: 22320202] |
| Pediatric Mephrology | Urinary angiotensinogen as a marker of intrarenal angiotensin II activity in adolescents with primary hypertension[PubMed: PMC3661928] |
| Acta Paediatrica | Urinary angiotensinogen as a novel marker of obstructive nephropathy in children[Pubmed: 23772991] |
| Journal of the Renin-Angiotensin-Aldosterone System | Association between urinary angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsia[Sagepub: Source] |
| Pediatric Nephrology | MP707 CHARACTERISTICS AND OUTCOMES OF CHILDREN WITH AUTOSOMAL RECESSIVE POLYCYSTIC KIDNEY DISEASE COHORT FROM THE ESPN/ERA-EDTA REGISTRY[Oxfordjournals:Source] |
| journal of human hypertension | Relationship between blood pressure var[PubMed: 26223347] |
| PLoS One | Higher Serum Angiotensinogen Is an Indicator of IgA Vasculitis with Nephritis Revealed by Comparative Proteomes Analysis[PubMed: 26098644] |
| MKA | Korelasi pengukuran antropometri dengan tekanan darah dan angiotensinogen plasma pada dewasa[Index.Php: Art] |
| Journal of Human Hypertension | Relationship between blood pressure variability and renal activity of the renin-angiotensin system.[pubmed:26223347] |
| Acta Pharmacol Sin. | Pharmacological evidence: a new therapeutic approach to the treatment of chronic heart failure through SUR2B/Kir6.1 channel in endothelial cells.[pubmed:27890915] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis[S0021967317311858] |
| Life Sciences | Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activity[Pubmed:30036488] |
| American Journal of Physiology-Renal Physiology | Role of (Pro) Renin Receptor in Albumin Overload-Induced Nephropathy in Rats[Pubmed:29846109] |
| Methods in Molecular Biology | Quantitation of Total and Free Thiol Angiotensinogen as a Prognostic Marker for Preeclampsia[Pubmed: 31069778] |
| J Transl Med | Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans[33407555] |
| Hypertens Res | Association of Ang-(1¨C7) and des-Arg 9 BK as new biomarkers of obesity and cardiometabolic risk factors in adolescents[33568792] |
| Physiol Rep | (Pro) renin receptor antagonist PRO20 attenuates nephrectomy©\induced nephropathy in rats via inhibition of intrarenal RAS and Wnt/¦Â©\catenin signaling[34057312] |
| Cancer Cell Int | A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer[34215253] |