膜联蛋白A1(ANXA1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Annexin A1 (ANXA1)

ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein

  • 膜联蛋白A1(ANXA1)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 膜联蛋白A1(ANXA1)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对膜联蛋白A1(ANXA1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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编号适用物种:Homo sapiens (Human,人)应用(仅供研究使用,不用于临床诊断!)
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参考文献

杂志参考文献
Plos oneDysregulation of Anti-Inflammatory Annexin A1 Expression in Progressive Crohns Disease[PubMed: PMC3794972]
African Journals OnlineModulatory Effect of Phytoestrogens and Curcumin on Induction of Annexin 1 in Human Peripheral Blood Mononuclear Cells and their Inhibitory Effect on Secretory Phospholipase A2[Ajol: Source]
Antimicrobial Agents and ChemotherapyHost Biomarkers of Invasive Pulmonary Aspergillosis to Monitor Therapeutic Response[Asm: Source]
J ProteomicsCharacterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC)[PubMed: 26718731]
DiabetesAnnexin A1 Is a Key Modulator of Mesenchymal Stromal Cell–Mediated Improvements in Islet Function[PubMed: 26470781]
PLoS OneAnnexin A1 Is Increased in the Plasma of Preeclamptic Women[PubMed: 26398190]
中国现代医学杂志 China Journal of Modern Medicine膜联蛋白A1 和CA19-9 在胆管癌中的表达及其临床意义[article:8686]
arteriosclerosis thrombosis and vascular biologyProtective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report.[pubmed:28062503]
Clinical ScienceMesenchymal stromal cells improve human islet function through released products and extracellular matrix.[pubmed:29101297]
Biomedicine & PharmacotherapyProresolving protein Annexin A1: The role in type 2 diabetes mellitus and obesity[Pubmed:29677533]
 International Journal of Chronic Obstructive Pulmonary DiseaseAnnexin A1 is elevated in patients with COPD and affects lung fibroblast function[Pubmed:29440885]
Medical MycologyApplying host disease status biomarkers to therapeutic response monitoring in invasive aspergillosis patients[Pubmed:29370415]
DEVELOPMENTAL NEUROPSYCHOLOGYEFFECTS OF MULTICOMPONENT EXERCISE ON PROGRESSION OF BRAIN ATROPHY AND COGNITION IN AMNESTIC MILD COGNITIVE IMPAIRMENT …[pdf:3395]
Multiple sclerosis and related disordersResolution of inflammation in neuromyelitis optica spectrum disorders[Pubmed: 30300851]
Acta PharmaceuticaEffects of annexin A1 on apoptosis and cell cycle arrest in human leukemic cell lines[]
npj Science of FoodAnnexin A1 accounts for an anti-inflammatory binding target of sesamin metabolites[Pubmed: 32133417]
FASEB JOURNALANXA1 directs Schwann cells proliferation and migration to accelerate nerve regeneration through the FPR2/AMPK pathway[Pubmed: 32908567]
Rev Soc Bras Med TropExpression of annexin-A1 in blood and tissue leukocytes of leprosy patients[33263684]
science advancesAnnexin-A1 SUMOylation regulates microglial polarization after cerebral ischemia by modulating IKK¦Á stability via selective autophagy[33523920]