武汉云克隆科技股份有限公司

载脂蛋白E(APOE)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Apolipoprotein E (APOE)

Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset

  • 载脂蛋白E(APOE)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 载脂蛋白E(APOE)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对载脂蛋白E(APOE)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种：Mus musculus (Mouse，小鼠)应用(仅供研究使用，不用于临床诊断！)
RPA704Mu02载脂蛋白E(APOE)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
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LMA704Mu载脂蛋白E(APOE)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSA704Mu01载脂蛋白E(APOE)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
Seizure - European Journal of EpilepsyCerebrospinal fluid apolipoprotein E concentration decreases after seizure[Seizure: PIIS1059131109002441]
Biochimica et Biophysica ActaHepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties[PubMed: 23328279 ]
Medical OncologyExpression and clinical significance of apolipoprotein E in pancreatic ductal adenocarcinoma[Pubmed: 23609192]
Diabetes.Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human Obesity[Pubmed:25249572]
renal failureFrequencies of apolipoprotein E alleles in depressed patients undergoing hemodialysis – a case-control study[PubMed: 25707516]
Biochim Biophys ActaCulturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism[PubMed: 26515253]
DiabetesAdipocyte ATP-binding cassette G1 promotes triglyceride storage, fat mass growth, and human obesity[PubMed: 25249572]
Scientific ReportsAdaptive immunity against gut microbiota enhances apoE-mediated immune regulation andreduces atherosclerosis and western-diet-related inflammation.[pubmed:27383250]
Biochemical Pharmacology Differential effects of apoE and apoJ mimetic peptides on the action of an anti-Aβ scFv in 3xTg-AD mice[Pubmed:30026023]
OncotargetDeletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse …[Pubmed:29731975]
Proteomics Clinical ApplicationsComparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy[Pubmed: 31162828]
Nature CommunicationsHepatic HuR modulates lipid homeostasis in response to high-fat diet[Pubmed: 32546794]
VETERINARY PATHOLOGYPathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats[Pubmed: 32880234]
Journal of Clinical MedicineThe Significance of Apolipoprotein E Measurement in the Screening of Fetal Down Syndrome[33321701]
Cancer Cell IntDiscovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer[33602229]
Nat CommunGP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity[34853313]
TalantaIridium nanoclusters as high sensitive-tunable elemental labels for immunoassays: Determination of IgE and APOE in aqueous humor by inductively coupled …[Pubmed:35378356]
Analytica Chimica ActaGold nanoclusters as elemental label for the sequential quantification of apolipoprotein E and metallothionein 2A in individual human cells of the retinal …[Pubmed:35361429]