精氨酸酶(ARG)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Arginase (ARG)

ARG1; Arginase I; Liver Arginase

特异性

试剂盒中的抗体对精氨酸酶(ARG)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种:Mus musculus (Mouse,小鼠)应用(仅供研究使用,不用于临床诊断!)
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参考文献

杂志参考文献
PLoS OnePhenobarbital Induces Alterations in the Proteome of Hepatocytes and Mesenchymal Cells of Rat Livers[Pubmed: 24204595]
J Cereb Blood Flow MetabArginase I release from activated neutrophils induces peripheral immunosuppression in a murine model of stroke[PubMed: 25966956]
Cancer MedicineCirculating CD14+HLA-DR-/low myeloid-derived suppressor cells in leukemia patients with allogeneic hematopoietic stem cell transplantation: novel clinical potential strategies for the prevention and cellular therapy of graft-versus-host disease[Pubmed:27109254]
Cell Mol Immunol Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica[Pubmed:27157496]
Potent but transient immunosuppression of T-cells is a general feature of erythroid progenitor cells[]
Commun BiolPotent but transient immunosuppression of T-cells is a general feature of CD71+ erythroid cells[34893694]