钙网蛋白(CALR)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Calreticulin (CALR)
CRT; RO; SSA; CC1qR; ERp60; HACBP; grp60; CRTC; CRP55; Calregulin; Sicca Syndrome Antigen A; Autoantigen Ro; Endoplasmic reticulum resident protein 60
- 编号KSB486Hu01
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.625-40ng/mL
- 可适灵敏度0.273ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7182¥ 11970¥ 21546¥ 41895¥ 71820
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
特异性
试剂盒中的抗体对钙网蛋白(CRT)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APB486Hu61 | 钙网蛋白(CALR)活性蛋白 | Cell culture; Activity Assays. |
| EPB486Hu61 | 钙网蛋白(CALR)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB486Hu03 | 钙网蛋白(CALR)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB486Hu02 | 钙网蛋白(CALR)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB486Hu01 | 钙网蛋白(CALR)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPB486Hu21 | 钙网蛋白(CALR)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAB486Hu02 | 钙网蛋白(CALR)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| PAB486Hu01 | 钙网蛋白(CALR)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| PAB486Hu08 | 钙网蛋白(CALR)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB486Hu71 | 钙网蛋白(CALR)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB486Hu22 | 钙网蛋白(CALR)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB486Hu | 钙网蛋白(CALR)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEB486Hu | 钙网蛋白(CALR)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB486Hu | 钙网蛋白(CALR)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB486Hu01 | 钙网蛋白(CALR)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Journal of Investigative Dermatology | Oxidative Stress-Induced Calreticulin Expression and Translocation: New Insights into the Destruction of Melanocytes[Pubmed: 23771121] |
| International Journal of Molecular Sciences | The Anticoagulant Effect of PGI2S and tPA in Transgenic Umbilical Vein Endothelial Cells Is Linked to Up-Regulation of PKA and PKC[Mdpi: Source] |
| The Journal of Maternal-Fetal & Neonatal Medicine | Amniotic fluid calreticulin in pregnancies complicated by the preterm prelabor rupture of membranes[Pubmed:26953684] |
| journal of maternal-fetal & neonatal medicine | Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes.[pubmed:28152632] |
| Talanta | Investigation of the interaction between calreticulin and immunoglobulin G by capillary electrophoresis and computer modeling[Doi: 10.1016/j.talanta.2018.11.098] |
| Sci Adv | Thermostable ionizable lipid-like nanoparticle (iLAND) for RNAi treatment of hyperlipidemia[Pubmed:35171673] |