胱天蛋白酶8(CASP8)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Caspase 8 (CASP8)
CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog
- 编号KSA853Mu01
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.312-20ng/mL
- 可适灵敏度0.117ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 6221¥ 10368¥ 18662¥ 36288¥ 62208
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特异性
试剂盒中的抗体对胱天蛋白酶8(CASP8)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA853Mu01 | 胱天蛋白酶8(CASP8)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA853Mu02 | 胱天蛋白酶8(CASP8)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA853Mu01 | 胱天蛋白酶8(CASP8)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA853Mu02 | 胱天蛋白酶8(CASP8)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA853Mu81 | 胱天蛋白酶8(CASP8)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA853Mu71 | 胱天蛋白酶8(CASP8)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA853Mu | 胱天蛋白酶8(CASP8)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA853Mu | 胱天蛋白酶8(CASP8)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA853Mu01 | 胱天蛋白酶8(CASP8)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Molecular Neurobiology | Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations[Pubmed:25502462] |
| Journal of Ethnopharmacology | Viscum articulatum Burm. f. aqueous extract exerts antiproliferative effect and induces cell cycle arrest and apoptosis in leukemia cells[Pubmed:29555410] |
| Clinical And Experimental Pharmacology And Physiology | Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway[Pubmed:29266336] |
| Environmental Toxicology | Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice[Pubmed: 31087429] |
| Environ Toxicol Pharmacol. | Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats[Pubmed: 31421311] |
| Journal of Biochemical and Molecular Toxicology | Tranilast attenuates methotrexate‐induced renal and hepatic toxicities: Role of apoptosis‐induced tissue proliferation[Pubmed: 32045101] |
| life sciences | Nifuroxazide attenuates experimentally-induced hepatic encephalopathy and the associated hyperammonemia and cJNk/caspase-8/TRAIL activation in rats[Pubmed: 32259601] |
| Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells[Pubmed: 33206337] |
| Int Immunopharmacol | Cardio-protective impact of gabapentin against doxorubicin-induced myocardial toxicity in rats; emphasis on modulation of inflammatory-apoptotic signaling[Pubmed: 33199237] |
| ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | Chemo-preventive effect of crocin against experimentally-induced hepatocarcinogenesis via regulation of apoptotic and Nrf2 signaling pathways[Pubmed: 32942000] |
| Environ Sci Pollut Res Int | Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low− dose of …[Pubmed: 32915453] |
| J Biochem Mol Toxicol | Tranilast abrogates cisplatin©\induced testicular and epididymal injuries: An insight into its modulatory impact on apoptosis/proliferation[34047436] |