高尔基磷酸化蛋白2(GOLPH2)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Golgi Phosphoprotein 2 (GOLPH2)
GOLM1; GP73; C9orf155; Golgi Membrane Protein 1; Golgi Protein 73
- 编号KSB668Mu01
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.94-60ng/mL
- 可适灵敏度0.37ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7387¥ 12312¥ 22162¥ 43092¥ 73872
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对高尔基蛋白73(GP73)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB668Mu02 | 高尔基磷酸化蛋白2(GOLPH2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB668Mu01 | 高尔基磷酸化蛋白2(GOLPH2)活性蛋白 | Cell culture; Activity Assays. |
| RPB668Mu01 | 高尔基磷酸化蛋白2(GOLPH2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB668Mu02 | 高尔基磷酸化蛋白2(GOLPH2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB668Mu01 | 高尔基磷酸化蛋白2(GOLPH2)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| LAB668Mu81 | 高尔基磷酸化蛋白2(GOLPH2)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAB668Mu71 | 高尔基磷酸化蛋白2(GOLPH2)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEB668Mu | 高尔基磷酸化蛋白2(GOLPH2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB668Mu | 高尔基磷酸化蛋白2(GOLPH2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB668Mu01 | 高尔基磷酸化蛋白2(GOLPH2)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Molecular & Cellular Proteomics | LAMC2: A promising new pancreatic cancer biomarker identified by proteomic analysis of pancreatic adenocarcinoma tissues[Mcponline: 023507] |
| Afro-Egypt J Infect Endem Dis | Diagnostic and Prognostic Validity of Serum Golgi Protein 73 in Egyptian Patients with Hepatocelluar Carcinoma[Ajied: Ajied_System_Files] |
| Journal of Applied Pharmaceutical Science | Diagnostic role of Golgi protein 73 and IL-17 in Egyptian cirrhotic rather than hepatocellular carcinoma patients.[handle:123456789] |
| Oncology Letters | Serum Golgi protein 73 is a prognostic rather than diagnostic marker in hepatocellular carcinoma[pubmed:29113278] |
| Scientific Reports | The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study[Pubmed: 32198440] |
| Hepatobiliary & Pancreatic Diseases International | Dynamic expression of hepatic GP73 mRNA and protein and circulating GP73 during hepatocytes malignant transformation[Pubmed: 32171652] |
| Biomolecules | Deoxycholic Acid Upregulates Serum Golgi Protein 73 through Activating NF-¦ÊB Pathway and Destroying Golgi Structure in Liver Disease. Biomolecules 2021, 11?¡[33540642] |
| ADVANCED RESEARCHES | Search for Effective Serum Tumor Markers for Early Diagnosis of Hepatocellular Carcinoma Associated with Hepatitis C[] |