70kDa热休克蛋白1A(HSPA1A)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Heat Shock 70kDa Protein 1A (HSPA1A)
HSP70-1; HSP-A1A; HSPA1; HSP70-2; HSX70
- 编号KSB081Bo01
- 物种Bos taurus; Bovine (Cattle，牛)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围1.56-100ng/mL
- 可适灵敏度0.61ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 8618¥ 14364¥ 25855¥ 50274¥ 86184
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对70kDa热休克蛋白1A(HSPA1A)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Bos taurus; Bovine (Cattle，牛) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB081Bo01 | 70kDa热休克蛋白1A(HSPA1A)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB081Bo01 | 70kDa热休克蛋白1A(HSPA1A)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB081Bo81 | 70kDa热休克蛋白1A(HSPA1A)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAB081Bo71 | 70kDa热休克蛋白1A(HSPA1A)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB081Bo21 | 70kDa热休克蛋白1A(HSPA1A)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB081Bo | 70kDa热休克蛋白1A(HSPA1A)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB081Bo | 70kDa热休克蛋白1A(HSPA1A)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB081Bo01 | 70kDa热休克蛋白1A(HSPA1A)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Journal of Neuroinflammation | Identification of potential biomarkers of gold nanoparticle toxicity in rat brains[PubMed: 22691312] |
| Mediators Inflamm.? | Local Inflammation in Fracture Hematoma: Results from a Combined Trauma Model in Pigs[Pubmed:Pmc4324980] |
| Eur J Pharmacol. | Differential modulatory effects of morphine on acute and chronic stress induced neurobehavioral and cellular markers in rats[Pubmed:24508522] |
| Pharmacol Rep. | Effect of propranolol on IL-10, visfatin, Hsp70, iNOS, TLR2, and survivin in amelioration of tumor progression and survival in Solid Ehrlich Carcinoma-bearing mice[Pubmed:25443743] |
| Advances in Medical Sciences | A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics[pubmed:28822267] |
| Archives of Medical Research | Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young …[Pubmed:29379533] |
| metabolic brain disease | Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampus[Pubmed: 32172517] |
| EXPERIMENTAL CELL RESEARCH | Antagomirs targeting miR-142–5p attenuate pilocarpine-induced status epilepticus in mice[Pubmed: 32439493] |