胰岛素样生长因子结合蛋白3(IGFBP3)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)

BP53; IBP3; Growth Hormone-Dependent Binding Protein; Acid Stable Subunit Of The 140 K IGF Complex

特异性

试剂盒中的抗体对胰岛素样生长因子结合蛋白3(IGFBP3)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种:Homo sapiens (Human,人)应用(仅供研究使用,不用于临床诊断!)
APA054Hu01胰岛素样生长因子结合蛋白3(IGFBP3)活性蛋白Cell culture; Activity Assays.
RPA054Hu01胰岛素样生长因子结合蛋白3(IGFBP3)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA054Hu03胰岛素样生长因子结合蛋白3(IGFBP3)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA054Hu02胰岛素样生长因子结合蛋白3(IGFBP3)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
EPA054Hu61胰岛素样生长因子结合蛋白3(IGFBP3)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
EPA054Hu62胰岛素样生长因子结合蛋白3(IGFBP3)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA054Hu02胰岛素样生长因子结合蛋白3(IGFBP3)活性蛋白Cell culture; Activity Assays.
PAA054Hu01胰岛素样生长因子结合蛋白3(IGFBP3)多克隆抗体WB; IHC; ICC; IP.
LAA054Hu71胰岛素样生长因子结合蛋白3(IGFBP3)多克隆抗体(生物素标记)WB; IHC; ICC.
MAA054Hu22胰岛素样生长因子结合蛋白3(IGFBP3)单克隆抗体WB; IHC; ICC; IP.
MAA054Hu25胰岛素样生长因子结合蛋白3(IGFBP3)单克隆抗体WB
MAA054Hu27胰岛素样生长因子结合蛋白3(IGFBP3)单克隆抗体WB; IHC; ICC; IP.
MAA054Hu29胰岛素样生长因子结合蛋白3(IGFBP3)单克隆抗体WB; IHC; ICC; IP.
MAA054Hu21胰岛素样生长因子结合蛋白3(IGFBP3)单克隆抗体WB
MAA054Hu23胰岛素样生长因子结合蛋白3(IGFBP3)单克隆抗体WB; IHC
MAA054Hu24胰岛素样生长因子结合蛋白3(IGFBP3)单克隆抗体WB
SEA054Hu胰岛素样生长因子结合蛋白3(IGFBP3)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA054Hu胰岛素样生长因子结合蛋白3(IGFBP3)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSA054Hu01胰岛素样生长因子结合蛋白3(IGFBP3)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
Molecular Biology ReportsCharacterization of the specific and sustained GH1 expression induced by rAAV2/1 in normal adult male rats[PubMed: 20204528]
EndocrinologyRole of IGFBP-3 in the Regulation of β-Cell Mass during Obesity: Adipose Tissue/β-Cell Cross Talk[Endo: source]
Nutrition and CancerA Pilot Study Comparing the Effect of Flaxseed, Aromatase Inhibitor, and the Combination on Breast Tumor Biomarkers[Tandfonline: Source]
Dig Dis SciThe Protective Effects of Exclusive Enteral Nutrition Formulas on Growth Factor Expression and the Proximal Tibial Epiphyseal Growth Plate in a TNBS-Induced IBD Rat Model[PubMed: 25708898]
Animal.Effects of a dietary crude fibre concentrate on growth in weaned piglets.[pubmed:28318464]
PLoS OneAltered insulin-like growth factor-2 signaling is associated with psychopathology and cognitive deficits in patients with schizophrenia[Pubmed: 32191705]
Int J Environ Res Public HealthAssociation of Salivary IGF and IGF/IGFBP-3 Molar Ratio with Cervical Vertebral Maturation Stages from Pre-Adolescent to Post-Adolescent Transition Period—A …[Pubmed:35564566]