层粘连蛋白(LN)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Laminin (LN)

LAM

    特异性

    试剂盒中的抗体对层粘连蛋白(LN)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

    用法

    1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
    2.洗板1次;
    3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
    4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

    储存

    抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

    配套试剂

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    参考文献

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