武汉云克隆科技股份有限公司

基质Gla蛋白(MGP)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Matrix Gla Protein (MGP)

MGLAP; GIG36; Cell growth-inhibiting gene 36 protein

  • 基质Gla蛋白(MGP)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 基质Gla蛋白(MGP)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对基质Gla蛋白(MGP)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

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参考文献

杂志参考文献
Nephrology Dialysis TransplantationMagnesium prevents phosphate-induced calcification in human aortic vascular smooth muscle cells[PubMed: PMC3611891]
Maturitas. Bone metabolism regulators and arterial stiffness in postmenopausal women[Pubmed: 23916080]
The Egyptian RheumatologistOsteoprotegerin (OPG) and Matrix Gla protein (MGP) in rheumatoid arthritis patients: Relation to disease activity[ScienceDirect: S1110116414000076]
Toxicology ReportsCanola and hydrogenated soybean oils accelerate ectopic bone formation induced by implantation of bone morphogenetic protein in mice[Researchgate:Source]
Lipids in Health and DiseaseMarkers of increased cardiovascular risk in patients with chronic kidney disease[Biomedcentral:Source]
Human & Veterinary MedicineThe behavior of circulating matrix Gla protein, matrix metalloproteinase-9 and nitrotyrosine in patients with varicose veins[Hvm:Source]
Pol Arch Med WewnEffect of Vitamin K2 on progression of atherosclerosis and vascular calcification in nondialyzed patients with chronic kidney disease stages 3-5[PubMed: 26176325]
Lipids Health Dis.Markers of increased atherosclerotic risk in patients with chronic kidney disease: a preliminary study[pmc:PMC4739105]
Kidney Blood Press Res.Plasma Desphospho-Uncarboxylated Matrix Gla Protein as a Marker of Kidney Damage and Cardiovascular Risk in Advanced Stage of Chronic Kidney Disease[Pubmed:27100101]
Archives of Oral BiologyMatrix-Gla Protein rs4236 [A/G] gene polymorphism and serum and GCF levels of MGP in patients with subgingival dental calculus[pubmed:27348051]
Cell Death & DiseaseMesenchymal stromal cells-derived matrix Gla protein contribute to the alleviation of experimental colitis[Pubmed:29880866]
UrologyHigh concentration of calcium promotes mineralization in NRK52E cells via inhibiting the expression of matrix Gla protein[Pubmed:29935264]
Clinica Chimica ActaSerum total matrix Gla protein: Reference interval in healthy adults and variations in patients with vascular and osteoarticular diseases[Pubmed: 30597137]
Biological Trace Element ResearchAltered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis Patients[Pubmed: 30847765]
Renal FailureRelationship of matrix Gla protein and vitamin K with vascular calcification in hemodialysis patients[Pubmed: 31538831]
HeliyonMatrix Gla protein maintains normal and malignant hematopoietic progenitor cells by interacting with bone morphogenetic protein-4[Pubmed: 32322728]