粘蛋白5B(MUC5B)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Mucin 5 Subtype B (MUC5B)
MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtype B,Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin
- 编号KSA684Mu01
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.625-40ng/mL
- 可适灵敏度0.255ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 6221¥ 10368¥ 18662¥ 36288¥ 62208
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对粘蛋白5B(MUC5B)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA684Mu02 | 粘蛋白5B(MUC5B)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA684Mu01 | 粘蛋白5B(MUC5B)活性蛋白 | Cell culture; Activity Assays. |
| RPA684Mu01 | 粘蛋白5B(MUC5B)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA684Mu01 | 粘蛋白5B(MUC5B)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA684Mu02 | 粘蛋白5B(MUC5B)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA684Mu81 | 粘蛋白5B(MUC5B)多克隆抗体(异硫氰酸荧光素标记) | Applications: WB; IHC; ICC; IP. |
| LAA684Mu71 | 粘蛋白5B(MUC5B)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA684Mu | 粘蛋白5B(MUC5B)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA684Mu | 粘蛋白5B(MUC5B)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA684Mu01 | 粘蛋白5B(MUC5B)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| The Journal of Biological Chemistry | The Extracellular Signal-regulated Kinase Mitogen-activated Protein Kinase/Ribosomal S6 Protein Kinase 1 Cascade Phosphorylates cAMP Response Element-binding Protein to Induce MUC5B Gene Expression via D-Prostanoid Receptor Signaling[Jcb: 34199] |
| The Journal of Biological Chemistry | The ERK MAPK/RSK1 cascade phosphorylates CREB to induce MUC5B gene expression via DP1 receptor signaling[Jcb: 247684] |
| Respiratory Researh | Pyocyanin-induced mucin production is associated with redox modification of FOXA2[PubMed: PMC3765780] |
| Respirology | Secreted mucins and airway bacterial colonization in non-CF bronchiectasis[PubMed: 26172851] |
| American Thoracic Society International Conference Abstracts | Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness[doi:10.1164] |
| Ann Am Thorac Soc | Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization[doi:10.1513] |
| Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой[10.20538/1682-0363-2017-2-146-158] | |
| Allergy | Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps[Pubmed:29802623] |
| European Journal of Pharmacology | Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling[Pubmed:29382534] |
| Japan Society of Immunology & Allergology in Otolaryngology | インフルエンザ菌Phosphorylcholine の表出と中耳ムチンMUC5AC 及びMUC5B 産生への影響[10.5648:jjiao.36.7] |
| Journal of Investigative Medicine | The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease[Pubmed: 30573494] |
| Mucosal Immunology | ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanism[Pubmed: 31278375] |