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神经营养因子3(NT3)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Neurotrophin 3 (NT3)

NTF3; HDNF; NGF2; Nerve Growth Factor 2; Neurotrophic Factor

  • 神经营养因子3(NT3)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 神经营养因子3(NT3)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对神经营养因子3(NT3)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
RPA106Hu01神经营养因子3(NT3)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
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SCA106Hu神经营养因子3(NT3)检测试剂盒(化学发光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMA106Hu神经营养因子3(NT3)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSA106Hu01神经营养因子3(NT3)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
SCaracteriza[Teses: Disponiveis]
Canadian Journal of Physiology and PharmacologyProtective effects of telmisartan and tempol on lipopolysaccharide-induced cognitive impairment, neuroinflammation, and amyloidogenesis: possible role of brain-derived neurotrophic factor[pubmed:28388365]
Efecto de Diferentes Intervenciones de Ejercicio Físico Agudo Sobre Niveles Plasmáticos de BDNF, NT-3 y NT-4 en Adultos con Sobrepeso[:]
Artificial Cells Nanomedicine and BiotechnologyHistological and functional outcomes in a rat model of hemisected spinal cord with sustained VEGF/NT-3 release from tissue-engineered grafts[Pubmed: 31899965]
laboratory investigationMacrophage-derived neurotrophin-3 promotes heterotopic ossification in rats[Pubmed: 31896816]
EUROPEAN CHILD & ADOLESCENT PSYCHIATRYIncreased serum brain-derived neurotrophic factor and adrenocorticotropic hormone levels are associated with obsessive compulsive disorder in medication?free?¡­[33389158]
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRYSea urchin gangliosides exhibit neuritogenic effects in neuronal PC12 cells via TrkA- and TrkB-related pathways[33589896]
Clin EpigeneticsGenome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism[34092241]
J Nutr Sci Vitaminol (Tokyo)Effects of a Tea Cultivar “MK5601” on Behaviors and Hippocampal Neurotrophin-3 Levels in Middle-Aged Mice[34193676]
Iranian Journal of Basic Medical SciencesAdrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons[34712429]
Molecular Biology ReportsUmbilical cord-derived mesenchymal stem cell conditioned medium reverses neuronal oxidative injury by inhibition of TRPM2 activation and the JNK signaling …[Pubmed:35585377]