脂质运载蛋白2(NGAL)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Neutrophil gelatinase-associated lipocalin (NGAL)
Lipocalin-2; LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2
- 编号KSB388Hu01
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.065ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 4838¥ 8064¥ 14515¥ 28224¥ 48384
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对中性粒细胞明胶酶关联脂质运载蛋白(NGAL)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB388Hu02 | 脂质运载蛋白2(NGAL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB388Hu61 | 脂质运载蛋白2(NGAL)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB388Hu01 | 脂质运载蛋白2(NGAL)活性蛋白 | Cell culture; Activity Assays. |
| RPB388Hu01 | 脂质运载蛋白2(NGAL)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB388Hu01 | 脂质运载蛋白2(NGAL)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB388Hu02 | 脂质运载蛋白2(NGAL)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB388Hu81 | 脂质运载蛋白2(NGAL)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAB388Hu71 | 脂质运载蛋白2(NGAL)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB388Hu23 | 脂质运载蛋白2(NGAL)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB388Hu26 | 脂质运载蛋白2(NGAL)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB388Hu27 | 脂质运载蛋白2(NGAL)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB388Hu28 | 脂质运载蛋白2(NGAL)单克隆抗体 | ICC/IF |
| MAB388Hu22 | 脂质运载蛋白2(NGAL)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB388Hu24 | 脂质运载蛋白2(NGAL)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB388Hu21 | 脂质运载蛋白2(NGAL)单克隆抗体 | WB |
| MAB388Hu25 | 脂质运载蛋白2(NGAL)单克隆抗体 | WB; IHC; ICC; IP. |
| FAB388Hu02 | 抗脂质运载蛋白2(NGAL)单克隆抗体 | Flow cytometry. |
| FAB388Hu04 | 抗脂质运载蛋白2(NGAL)单克隆抗体 | Flow cytometry. |
| SEB388Hu | 脂质运载蛋白2(NGAL)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB388Hu | 脂质运载蛋白2(NGAL)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB388Hu | 脂质运载蛋白2(NGAL)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSB388Hu01 | 脂质运载蛋白2(NGAL)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSB388Hu01 | 脂质运载蛋白2(NGAL)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| International Journal of Nephrology and Renovascular Disease | Correlation of serum neutrophil gelatinase-associated lipocalin with acute kidney injury in hypertensive disorders of pregnancy[PubMed: PMC3794962] |
| International Journal of Endocrinology | Cholesterol Contributes to Diabetic Nephropathy through SCAP-SREBP-2 Pathway[Pubmed: 24369464] |
| International Journal of Sport Studies | Lipocalin-2: Response To Resistance Training in Obese Young Men[Ijssjournal: Source] |
| Asian Journal of Sports Medicine | Effects of Resistance versus Endurance Training on Plasma Lipocalin-2 in Young Men[Tums: Source] |
| European Respiratory Journal | Differences in plasma and sputum biomarkers between COPD and COPD-asthma overlap[Pubmed: 23794464] |
| Acta Histochem.? | Suppressor of cytokine signaling (SOCS) 2 attenuates renal lesions in rats with diabetic nephropathy[Pubmed:24802166] |
| Int Urol Nephrol. | Nephrotoxic effects of varenicline as the most effective drug used for smoking cessation: a preliminary experimental study[Pubmed:25812822] |
| 世界华人消化杂志 | 血清及胆汁中NGAL 对于区分良恶性胆道狭窄的临床意义[Wjgnet:Source] |
| Diabetes & Vascular Disease Research | Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats[Sagepub:Source] |
| Biomedical & Pharmacology Journal | Hs-CRP and Adipokin (Lcn2): Response to Exercise Training in Obese Men[Biomedpharmajournal:Source] |
| Asian Journal of Physical Education and Computer Science in Sports | A Study On Thyroid Hormone And Its Effects On Exercise [Ifcss:Source] |
| 临床儿科杂志 | 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义[Jcp-Sh:Source] |
| Correlation of serum neutrophil gelatinase associated lipocalin with disease severity in hypertensive disorders of pregnancy[Pubmed:Pmc4260273] | |
| Int J Chron Obstruct Pulmon Dis. | Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary disease[Pubmed:Pmc4043430] |
| Pediatric Anesthesia? | Impact of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal model[Pubmed:24916275] |
| Inflammation.? | Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis[Pubmed:25448261] |
| Int J Clin Exp Pathol | Rosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitis[PubMed: 26191125] |
| BMC Anesthesiol | Increased cardiac index attenuates septic acute kidney injury: a prospective observational study[PubMed: 25745357] |
| 中华临床医生杂志 | 中性粒细胞明胶酶相关脂质运载蛋白对腹膜透析相关性腹膜炎疗效监测的意义[Journal: Fulltext] |
| PLoS One | Ameliorative Effect of Chrysin on Adenine-Induced Chronic Kidney Disease in Rats[PubMed: 25909514] |
| Pediatric Anesthesia | Impact of high doses of 6% hydroxyethyl starch 130/0.42 and 4% gelatin on renal function in a pediatric animal model[Pubmed:26714833] |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.[Pubmed:26991748] |
| Archives of Biological Sciences | Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol[publication:299492265_Diabetes-induced_renal_failu] |
| International Journal of Molecular Medicine | The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats[pubmed:27430552] |
| Hepatology. | Hyperoxidized albumin modulates neutrophils to induce oxidative stress and inflammation in severe alcoholic hepatitis.[pubmed:27775820] |
| Lipids in Health and Disease | Advanced glycation end products (AGEs) increase renal lipid accumulation: a pathogenic factor of diabetic nephropathy (DN)[pubmed:28659153] |
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases[pubmed:26991748] |
| Clin Exp Pharmacol Physiol. | Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats.[pubmed:29027259] |
| Scientific Reports | Hemojuvelin Predicts Acute Kidney Injury and Poor Outcomes Following Cardiac Surgery[Pubmed:29386545] |
| Life Sciences | Pyrrolidine dithiocarbamate inhibits mouse acute kidney injury induced by diclofenac by targeting oxidative damage, cytokines and NF-κB activity[Pubmed:30036488] |
| Molecules | Effect of Pinocembrin Isolated from Mexican Brown Propolis on Diabetic Nephropathy[Pubmed:29642511] |
| American Journal of Translational Research | Improved approach for normothermic machine perfusion of cold stored kidney grafts[Pubmed:30018731] |
| Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypes[ISBN:978-951-51-4039-5] | |
| Disease Markers | Potential Novel Biomarkers of Obstructive Nephropathy in Children with Hydronephrosis[Doi: 10.1155/2018/1015726] |
| Kidney and Blood Pressure Research | The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy[Pubmed: 30504713] |
| BMC Complementary and Alternative Medicine | Dual actions on gout flare and acute kidney injury along with enhanced renal transporter activities by Yokuininto, a Kampo medicine[Pubmed: 30871515] |
| Naunyn-Schmiedeberg's archives of pharmacology | Piceatannol protects against cisplatin nephrotoxicity via activation of Nrf2/HO-1 pathway and hindering NF-κB inflammatory cascade[Pubmed: 31197431] |
| Diabetes, Metabolic Syndrome and Obesity | A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation[Pubmed: 31447572] |
| Environment International | Toxicity assessment due to prenatal and lactational exposure to lead, cadmium and mercury mixtures[Pubmed: 31639605] |
| Scientific Reports | Alkaline phosphatase treatment of Acute Kidney injury in an infant piglet Model of cardiopulmonary Bypass with Deep Hypothermic circulatory Arrest[Pubmed: 31578351] |
| Int Urol Nephrol | The evaluation of acute kidney injury due to ischemia by urinary neutrophil gelatinase-induced lipocalin (uNGAL) measurement in patients who underwent partial …[Pubmed: 32980930] |
| human & experimental toxicology | Upregulated miR-101 inhibits acute kidney injury–chronic kidney disease transition by regulating epithelial–mesenchymal transition[Pubmed: 32633566] |
| INTERNATIONAL JOURNAL OF ENDOCRINOLOGY | CОСТОЯНИЕ ТИРЕОИДНОЙ ФУНКЦИИ У ПАЦИЕНТОВ С САХАРНЫМ ДИАБЕТОМ 1-ГО ТИПА И ХРОНИЧЕСКОЙ БОЛЕЗНЬЮ ПОЧЕК …[] |
| INTERNATIONAL JOURNAL OF ENDOCRINOLOGY | Thyroid function in patients with type 1 diabetes mellitus and chronic kidney disease receiving renal replacement therapy[] |
| Pediatr Int | Early Kidney Injury in Immunoglobulin A Vasculitis: Role of Renal Biomarkers[33423326] |
| ARCHIVES OF TOXICOLOGY | Nrf2 deficiency aggravates the kidney injury induced by subacute cadmium exposure in mice[33398418] |
| Biomed Res Int | Renal Protective Effect of Beluga Lentil Pretreatment for Ischemia-Reperfusion Injury[33604384] |
| International Journal of Medical Sciences | Elevated plasma level of neutrophil gelatinase-associated lipocalin (NGAL) in patients with breast cancer[34104101] |
| Intensive Care Medicine Experimental | Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental …[34169407] |
| Biomolecules | Preservation of Mitochondrial Coupling and Renal Function by Controlled Oxygenated Rewarming of Porcine Kidney Grafts[34944524] |
| RSC Adv | SERS based Y-shaped aptasensor for early diagnosis of acute kidney injury[Pubmed:35733690] |