骨粘连蛋白(ON)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Osteonectin (ON)
SPARC; BM-40; Secreted Protein,Acidic,Cysteine-Rich; Basement-membrane protein 40; Secreted protein acidic and rich in cysteine
- 编号KSA791Mu01
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.059ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 6998¥ 11664¥ 20995¥ 40824¥ 69984
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
特异性
试剂盒中的抗体对骨粘连蛋白(ON)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA791Mu01 | 骨粘连蛋白(ON)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA791Mu02 | 骨粘连蛋白(ON)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA791Mu01 | 骨粘连蛋白(ON)多克隆抗体 | WB,IHC |
| PAA791Mu02 | 骨粘连蛋白(ON)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA791Mu81 | 骨粘连蛋白(ON)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAA791Mu71 | 骨粘连蛋白(ON)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA791Mu | 骨粘连蛋白(ON)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA791Mu | 骨粘连蛋白(ON)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA791Mu01 | 骨粘连蛋白(ON)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| J Mol Med (Berl) | Growth hormone replacement therapy regulates microRNA-29a and targets involved in insulin resistance[PubMed: 26199111] |
| Muscle Nerve | Effect of resistance ladder training on SPARC expression in skeletal muscle of hindlimb immobilized rats[PubMed: 26467437] |
| Medicine (Baltimore) | An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer[PubMed: 26266370] |
| British Journal of Cancer | A proteomics-based approach identifies secreted protein acidic and rich in cysteine as a prognostic biomarker in malignant pleural mesothelioma[Pubmed:26889976] |
| Journal of diabetes and its complications | Associations between FGF21, osteonectin and bone turnover markers in type 2 diabetic patients with albuminuria.[pubmed:27916484] |
| American journal of the medical sciences | Association of Bone Metabolic Markers With Diabetic Retinopathy and Diabetic Macular Edema in Elderly Chinese Individuals With Type 2 Diabetes Mellitus[pubmed:29078839] |
| Translational Research in Oral Oncology | Testican 1 (SPOCK1) and protein tyrosine phosphatase, receptor type S (PTPRS) show significant increase in saliva of tobacco users with oral cancer[Doi: 10.1177/2057178X18800534] |
| [ENGINEERING OF BIOMATERIALS | Osteoconductive potential of PLGA/bioglass composite biomaterials[] |