过氧化物酶体增殖物激活受体γ(PPARg)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Peroxisome Proliferator Activated Receptor Gamma (PPARg)
PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3
- 编号KSA886Mu01
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围0.156-10ng/mL
- 可适灵敏度0.065ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 6998¥ 11664¥ 20995¥ 40824¥ 69984
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
特异性
试剂盒中的抗体对过氧化物酶体增殖物激活受体γ(PPARg)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA886Mu03 | 过氧化物酶体增殖物激活受体γ(PPARg)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA886Mu01 | 过氧化物酶体增殖物激活受体γ(PPARg)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA886Mu02 | 过氧化物酶体增殖物激活受体γ(PPARg)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA886Mu03 | 过氧化物酶体增殖物激活受体γ(PPARg)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA886Mu02 | 过氧化物酶体增殖物激活受体γ(PPARg)多克隆抗体 | WB,ICC/IF |
| PAA886Mu01 | 过氧化物酶体增殖物激活受体γ(PPARg)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA886Mu71 | 过氧化物酶体增殖物激活受体γ(PPARg)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA886Mu | 过氧化物酶体增殖物激活受体γ(PPARg)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA886Mu | 过氧化物酶体增殖物激活受体γ(PPARg)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA886Mu01 | 过氧化物酶体增殖物激活受体γ(PPARg)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Neuroscience Letters | Elevated levels of PPAR-gamma in the cerebrospinal fluid of patients with multiple sclerosis[Pubmed: 24021801] |
| J Cell Biochem. | Nonivamide enhances miRNA let‐7d expression and decreases adipogenesis PPARγ expression in 3T3‐L1 cells[Pubmed:25704235] |
| Osteoarthritis Cartilage | Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone[PubMed: 26321377] |
| J Clin Diagn Res. | Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic Rats Supplemented with Momordica charantia[pmc:PMC4866090] |
| Journal of Clinical&Diagnostic Research | Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic RatsSupplemented with Momordica charantia.[pubmed:27190792] |
| Osteoarthritis and Cartilage | Establishment of a rabbit model to study the influence of advanced glycation end productsaccumulation on osteoarthritis and the protective effect of pioglitazone.[pubmed:26321377] |
| Neuroscience Letters | Unlike PPARgamma, neither other PPARs nor PGC-1alpha is elevated in the cerebrospinal fluid of patients with multiple sclerosis[pubmed:28483651] |
| European Journal of Immunology | Engulfment of Hb‐activated platelets differentiates monocytes into pro‐inflammatory macrophages in PNH patients[Pubmed:29677388] |
| Histochemistry and Cell Biology | Simpson–Golabi–Behmel syndrome human adipocytes reveal a changing phenotype throughout differentiation[Pubmed:29574488] |
| molecular and cellular biochemistry | Maternal omega-3 fatty acids and vitamin E improve placental angiogenesis in late-onset but not early-onset preeclampsia[Pubmed: 31420792] |
| Nutrients. | Hyperglycemia Changes Expression of Key Adipogenesis Markers (C/EBPα and PPARᵞ) and Morphology of Differentiating Human Visceral Adipocytes[Pubmed: 31398873] |
| journal of biochemical and molecular toxicology | Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cells[Pubmed: 31916655] |
| J Cosmet Dermatol | In©\vitro effect of pine bark extract on melanin synthesis, tyrosinase activity, production of endothelin©\1 and PPAR in cultured melanocytes exposed to Ultraviolet?¡[33960120] |
| American Chemical Society | TRPA1 Agonist Cinnamaldehyde Decreases Adipogenesis in 3T3-L1 Cells More Potently than the Non-agonist Structural Analog Cinnamyl Isobutyrate[33403292] |
| PLoS One | Quantitative real-time measurement of endothelin-1-induced contraction in single non-activated hepatic stellate cells[34343209] |
| Prostaglandins Leukot Essent Fatty Acids | Maternal Vitamin D Deficiency Reduces Docosahexaenoic Acid, Placental Growth Factor and Peroxisome Proliferator Activated Receptor Gamma levels in the Pup …[34768025] |
| Food and Chemical Toxicology | Melatonin attenuates cisplatin-induced acute kidney injury in mice: Involvement of PPARα and fatty acid oxidation[Pubmed:35367536] |