S100钙结合蛋白A6(S100A6)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for S100 Calcium Binding Protein A6 (S100A6)

S100-A6; 2A9; 5B10; CABP; CACY; PRA; MLN 4; Calcyclin; Growth factor-inducible protein 2A9; Prolactin receptor-associated protein

特异性

试剂盒中的抗体对S100钙结合蛋白A6(S100A6)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种:Rattus norvegicus (Rat,大鼠)应用(仅供研究使用,不用于临床诊断!)
RPB769Ra01S100钙结合蛋白A6(S100A6)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAB769Ra01S100钙结合蛋白A6(S100A6)多克隆抗体WB,IHC
LAB769Ra71S100钙结合蛋白A6(S100A6)多克隆抗体(生物素标记)WB; IHC; ICC.
MAB769Ra21S100钙结合蛋白A6(S100A6)单克隆抗体WB; IHC; ICC; IP.
SEB769RaS100钙结合蛋白A6(S100A6)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMB769RaS100钙结合蛋白A6(S100A6)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSB769Ra01S100钙结合蛋白A6(S100A6)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
Journal of ProteomicsTear proteome and protein network analyses reveal a novel pentamarker panel for tear film characterization in dry eye and meibomian gland dysfunction[PubMed: 23201116]
Tumour BiolDiagnostic significance of S100A2 and S100A6 levels in sera of patients with non-small cell lung cancer[PubMed: 26361956]
Clinical and Translational MedicineSerum levels of S100A6 are unaltered in patients with resectable cholangiocarcinoma[pubmed:27709523]
GastroenterologyTu1938-Micronutrients Assessment in Patients with Cirrhosis[10.1016:S0016-5085(18)33544-3]
GastroenterologyTu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis[10.1016:S0016-5085(18)33544-5]
Tumor BiologyProteomics detection of S100A6 in tumor tissue interstitial fluid and evaluation of its potential as a biomarker of cholangiocarcinoma[Pubmed:29629840]
scandinavian journal of clinical & laboratory investigationSerum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study[Pubmed: 31856598]
CELL BIOCHEMISTRY AND FUNCTIONS100A6 represses Calu©\6 lung cancer cells growth via inhibiting cell proliferation, migration, invasion and enhancing apoptosis[34008212]
MOLECULAR VISIONQuantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays[34012227]