S100钙结合蛋白A9(S100A9)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for S100 Calcium Binding Protein A9 (S100A9)
MRP14; MIF; 60B8AG; CFAG; NIF; CAGB; CGLB; L1AG; P14; Calgranulin B; Migration inhibitory factor-related protein 14; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain
- 编号KSB793Hu01
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围62.5-1000pg/mL
- 可适灵敏度27.1pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7182¥ 11970¥ 21546¥ 41895¥ 71820
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对S100钙结合蛋白A9(S100A9)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| APB793Hu01 | S100钙结合蛋白A9(S100A9)活性蛋白 | Cell culture; Activity Assays. |
| RPB793Hu01 | S100钙结合蛋白A9(S100A9)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB793Hu01 | S100钙结合蛋白A9(S100A9)多克隆抗体 | WB; IHC; ICC; IP. |
| LAB793Hu81 | S100钙结合蛋白A9(S100A9)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAB793Hu71 | S100钙结合蛋白A9(S100A9)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAB793Hu29 | S100钙结合蛋白A9(S100A9)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB793Hu22 | S100钙结合蛋白A9(S100A9)单克隆抗体 | WB; IHC |
| MAB793Hu21 | S100钙结合蛋白A9(S100A9)单克隆抗体 | WB; IHC |
| MAB793Hu23 | S100钙结合蛋白A9(S100A9)单克隆抗体 | WB |
| MAB793Hu27 | S100钙结合蛋白A9(S100A9)单克隆抗体 | WB; IHC; ICC; IP. |
| MAB793Hu24 | S100钙结合蛋白A9(S100A9)单克隆抗体 | WB; IHC |
| MAB793Hu25 | S100钙结合蛋白A9(S100A9)单克隆抗体 | WB; IHC; ICC; IP. |
| LAB793Hu82 | S100钙结合蛋白A9(S100A9)单克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAB793Hu72 | S100钙结合蛋白A9(S100A9)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEB793Hu | S100钙结合蛋白A9(S100A9)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB793Hu | S100钙结合蛋白A9(S100A9)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB793Hu01 | S100钙结合蛋白A9(S100A9)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| J. Proteome Res. | Differential Proteomic Analysis Distinguishes Tissue Repair Biomarker Signatures in Wound Exudates Obtained from Normal Healing and Chronic Wounds[Pubmed: source] |
| Biochemical and Biophysical Research Communications | High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese mice[ScienceDirect: S0006291X1200352X] |
| PLoS One. | Proteins with Altered Levels in Plasma from Glioblastoma Patients as Revealed by iTRAQ-Based Quantitative Proteomic Analysis[PubMed: 23029420] |
| Mainz | Identifizierung neuer potentieller Biomarker für das Kolonkarzinom sowie dessen Vorstufen[Info:Source] |
| PLOS ONE | Susceptibility to COPD: Differential Proteomic Profiling after Acute Smoking[Pubmed:25036363] |
| the faseb journal | Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation[PubMed: 25934702] |
| Sci Rep. | S100A9 and ORM1 serve as predictors of therapeutic response and prognostic factors in advanced extranodal NK/T cell lymphoma patients treated with pegaspargase/gemcitabine[pmc:PMC4810364] |
| Journal of Alzheimer's Disease | Proteomics Analysis of Blood Serums from Alzheimer's Disease Patients Using iTRAQ LabelingTechnology.[pubmed:27911324] |
| International journal of molecular sciences | Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds.[pubmed:28672877] |
| Gastroenterology | Tu1938-Micronutrients Assessment in Patients with Cirrhosis[10.1016:S0016-5085(18)33544-3] |
| Gastroenterology | Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis[10.1016:S0016-5085(18)33544-5] |
| Journal of Biological Chemistry | Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation[Pubmed:29496993] |
| Cancer Management and Research | The role of calgranulin B gene on the biological behavior of squamous cervical cancer in vitro and in vivo[Pubmed:29497331] |
| European review for medical and pharmacological sciences | S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα.[Pubmed:29949169] |
| Estudio de células inmunosupresoras y biomarcadores circulantes en carcinoma renal[] | |
| Lung | Mass Spectrometry Analysis of the Exhaled Breath Condensate and Proposal of Dermcidin and S100A9 as Possible Markers for Lung Cancer Prognosis[Pubmed: 31115649] |
| 中国医药导报 | 降钙素原, 超敏C 反应蛋白, 红细胞沉降率及S100A9 对肺结核合并肺部细菌感染的影响[] |
| scandinavian journal of clinical & laboratory investigation | Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study[Pubmed: 31856598] |
| PLoS One | Adverse reproductive effects of S100A9 on bovine sperm and early embryonic development in vitro[Pubmed: 31945120] |
| Sci Rep | Overexpression of S100A9 in tumor stroma contribute to immune evasion of NK/T cell lymphoma and predict poor response rate[34045609] |
| university of copenhagen | Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in[] |