转甲状腺素蛋白(PALB)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Prealbumin (PALB)

TBPA; PA; PLB; ATTR; TTR; Transthyretin; Prealbumin Amyloidosis Type I; Transports Thyroxine and Retinol

特异性

试剂盒中的抗体对转甲状腺素蛋白(TTR)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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参考文献

杂志参考文献
Chinese Medical JournalScreening disease-associated proteins from sera of patients with rheumatoid arthritis: a comparative proteomic study[PubMed: 20367977]
Journal of Vascular SurgeryCirculation levels of acute phase proteins in patients with Takayasu arteritis[PubMed: 20100644]
Journal of Pharmaceutical and Biomedical AnalysisEffect of high dose thiamine on the levels of urinary protein biomarkers in diabetes mellitus type 2[PubMed: 21130593]
Toxicology LettersSerum proteomic analysis reveals potential serum biomarkers for occupational medicamentosa-like dermatitis caused by trichloroethylene[Pubmed:24960064]
Journal of NeurochemistrySchwann cells contribute to neurodegeneration in transthyretin amyloidosis[Pubmed:25693163]
Anal BiochemSurface plasmon resonance assay of inhibition by pharmaceuticals for thyroxine hormone binging to transport proteins[PubMed: 26384643]
United States Patent ApplicationCompositions and Methods for Diagnosing Lung Cancer[y2016:0077095.html]
NeurotherapeuticsiTRAQ-Based Quantitative Proteomics Reveals the New Evidence Base for Traumatic Brain Injury Treated with Targeted Temperature Management[pubmed:29247448]
NeuroimmunomodulationCD4+ T Cells Affect the Thyroid Hormone Transport at the Choroid Plexus in Mice Raised in Enriched Environment[Pubmed: 30703773]
DiagnosticsSystemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J[Pubmed: 32585848]
Circulation-Genomic and Precision MedicinePrimary Cardiac Involvement in the Rare Transthyretin Ile73Val Mutation: A Case Series[Pubmed: 32393063]