泛素羧基端酯酶L1(UCHL1)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)

PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1

  • 泛素羧基端酯酶L1(UCHL1)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 泛素羧基端酯酶L1(UCHL1)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对泛素羧基端酯酶L1(UCHL1)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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编号适用物种:Homo sapiens (Human,人)应用(仅供研究使用,不用于临床诊断!)
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参考文献

杂志参考文献
J Neurol SciChanges of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesions[PubMed: 26232084]
Journal of the Neurological SciencesIncreased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcome[Pubmed:26810533]
Ulutas Medical JournalSerum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy [mnstemps:135]
Scientific ReportsActivation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytes[pubmed:28667290]
Intensive Care MedicineRelationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disability[Pubmed:29523900]
Earth and Environmental ScienceSerum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injury[article:10.1088]
Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhage[ISBN:978-952-03-0750-9]
Journal of Critical CareAssociation of neuronal repair biomarkers with delirium among survivors of critical illness[Pubmed: 31896448]
Brain SciencesBDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head Trauma[Pubmed: 32987792]