血管内皮生长因子C(VEGFC)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Vascular Endothelial Growth Factor C (VEGFC)

VEGF-C; Flt4-L; VRPL; Vascular Endothelial Growth Factor-Related Protein

特异性

试剂盒中的抗体对血管内皮生长因子C(VEGFC)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号适用物种:Homo sapiens (Human,人)应用(仅供研究使用,不用于临床诊断!)
APA145Hu61血管内皮生长因子C(VEGFC)活性蛋白Cell culture; Activity Assays.
RPA145Hu01血管内皮生长因子C(VEGFC)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA145Hu02血管内皮生长因子C(VEGFC)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
EPA145Hu62血管内皮生长因子C(VEGFC)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
EPA145Hu63血管内皮生长因子C(VEGFC)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
EPA145Hu61血管内皮生长因子C(VEGFC)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA145Hu01血管内皮生长因子C(VEGFC)活性蛋白Cell culture; Activity Assays.
PAA145Hu01血管内皮生长因子C(VEGFC)多克隆抗体WB; IHC; ICC; IP.
MAA145Hu23血管内皮生长因子C(VEGFC)单克隆抗体IHC
MAA145Hu24血管内皮生长因子C(VEGFC)单克隆抗体IHC
MAA145Hu22血管内皮生长因子C(VEGFC)单克隆抗体WB; IHC; ICC; IP.
MAA145Hu21血管内皮生长因子C(VEGFC)单克隆抗体IHC
MEA145Hu血管内皮生长因子C(VEGFC)检测试剂盒(酶联免疫吸附试验法,小样本)Enzyme-linked immunosorbent assay for Antigen Detection.
SEA145Hu血管内皮生长因子C(VEGFC)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA145Hu血管内皮生长因子C(VEGFC)检测试剂盒(化学发光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMA145Hu血管内皮生长因子C(VEGFC)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSA145Hu01血管内皮生长因子C(VEGFC)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
Journal of Cellular BiochemistryAlternatively activated RAW264. 7 macrophages enhance tumor lymphangiogenesis in mouse lung adenocarcinoma[PubMed: 19241443]
genetics and molecular researchExpression of COX-2 and VEGF-C in cholangiocarcinomas at different clinical and pathological stages[PubMed: 26125824]
Experimental and TherapeuticFenofibrate inhibits the expression of VEGFC and VEGFR-3 in retinal pigmental epithelial cells exposed to hypoxia[PubMed: 26622498]
Am J PatholIL-10 Indirectly Regulates Corneal Lymphangiogenesis and Resolution of Inflammation via Macrophages[PubMed: 26608451]
PLoS One.Interleukin-6 Induces Vascular Endothelial Growth Factor-C Expression via Src-FAK-STAT3 Signaling in Lymphatic Endothelial Cells.[pubmed:27383632]
Oncology LettersRNAi-mediated gene silencing of vascular endothelial growth factor C suppresses growth andinduces apoptosis in mouse breast cancer in vitro and in vivo.[pubmed:27895746]
AngiogenesisCharacterization of isolated liver sinusoidal endothelial cells for liver bioengineering[Pubmed:29582235]
Oncology ReportsEffects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cells[Pubmed:29328465]
LABORATORY INVESTIGATIONDynamic signature of lymphangiogenesis during acute kidney injury and chronic kidney disease[Pubmed: 31019289]
Cutaneous and Ocular ToxicologyIsotretinoin does not alter VEGF-A and VEGF-C levels: Do retinoids behave differently in dose-dependent and/or in vivo/in vitro conditions?[Pubmed: 32722957]