维生素D结合蛋白(DBP)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Vitamin D Binding Protein (DBP)
GC; VDBG; VDBP; Group-Specific Component; Gc-globulin
- 编号KSB810Ra01
- 物种Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围3.12-200ng/mL
- 可适灵敏度1.42ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7798¥ 12996¥ 23393¥ 45486¥ 77976
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对维生素D结合蛋白(DBP)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB810Ra02 | 维生素D结合蛋白(DBP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB810Ra01 | 维生素D结合蛋白(DBP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB810Ra02 | 维生素D结合蛋白(DBP)多克隆抗体 | WB; IHC |
| PAB810Ra01 | 维生素D结合蛋白(DBP)多克隆抗体 | WB; IHC |
| LAB810Ra71 | 维生素D结合蛋白(DBP)多克隆抗体(生物素标记) | WB |
| MAB810Ra25 | 维生素D结合蛋白(DBP)单克隆抗体 | ICC/IF |
| MAB810Ra26 | 维生素D结合蛋白(DBP)单克隆抗体 | WB |
| MAB810Ra24 | 维生素D结合蛋白(DBP)单克隆抗体 | ICC/IF |
| MAB810Ra22 | 维生素D结合蛋白(DBP)单克隆抗体 | WB; IHC |
| MAB810Ra21 | 维生素D结合蛋白(DBP)单克隆抗体 | WB; IHC |
| MAB810Ra23 | 维生素D结合蛋白(DBP)单克隆抗体 | WB,IHC,ICC/IF |
| FAB810Ra02 | 抗维生素D结合蛋白(DBP)单克隆抗体 | FCM |
| FAB810Ra04 | 抗维生素D结合蛋白(DBP)单克隆抗体 | FCM |
| FAB810Ra06 | 抗维生素D结合蛋白(DBP)单克隆抗体 | FCM |
| FAB810Ra08 | 抗维生素D结合蛋白(DBP)单克隆抗体 | FCM |
| SEB810Ra | 维生素D结合蛋白(DBP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB810Ra | 维生素D结合蛋白(DBP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB810Ra01 | 维生素D结合蛋白(DBP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Proteome Science | Isotope Coded Protein Labeling analysis of plasma specimens from acute severe dengue fever patients[PubMed: 23101585] |
| Toxins (Basel). | Increased Circulating Levels of Vitamin D Binding Protein in MS Patients[Pubmed:25590278] |
| Endocrine | Vitamin D-binding protein and free vitamin D concentrations in acromegaly[PubMed: 26547217] |
| Proteomics Clinical Applications | Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients[Pubmed:26680509] |
| PLoS One. | 25-Hydroxivitamin D Serum Concentration, Not Free and Bioavailable Vitamin D, Is Associated with Disease Activity in Systemic Lupus Erythematosus Patients.[pubmed:28085957] |
| PROTEOMICS - Clinical Applications | Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients.[pubmed:26680509] |
| Plos One | 25-Hydroxivitamin D serum concentration, not free and bioavailable vitamin D, is associated with disease activity in systemic lupus erythematosus patients[10.1371/journal.pone.0170323] |
| Nutrients | Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after …[Pubmed:30037068] |
| BBA- Molecular Basis of Disease | Enhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic …[Pubmed: 30553018] |
| J Clin Res Pediatr Endocrinol | Is Bioavailable Vitamin D Better Than Total Vitamin D to Evaluate Vitamin D Status in Obese Children?[34013709] |
| PLoS One | Leukocyte telomere length as a compensatory mechanism in vitamin D metabolism[Pubmed:35202418] |