组织蛋白酶G(CTSG)活性蛋白
Active Cathepsin G (CTSG)
CG; CTS-G
产品包装(模拟)
产品包装(模拟)
Figure. SDS-PAGE
通过ISO 9001、ISO 13485质量体系认证
活性实验
Cathepsin G (CTSG) is a neutral proteinase secreted by neutrophil granulocytes. It belongs to the family of serine proteases. Cathepsin G is a glycoprotein, which contains 1% carbohydrate. The activity assay of recombinant rat CTSG was measured by its ability to cleave the peptide substrate Suc-Ala-Ala Pro-Phe-pNA. The reaction was performed in 160 mM Tris-HCl, 1.6 M NaCl, pH 7.4 (Assay Buffer). The CTSG was diluted to different concentrations and the reaction was initiated by adding 50 ul CTSG to 50 ul 1 mM substrate and then read at 405 nm in kinetic mode for 5 minutes. The specific activity of recombinant rat CTSG is >70 pmol/min/µg.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APD993Ra01 | 组织蛋白酶G(CTSG)活性蛋白 | Cell culture; Activity Assays. |
| RPD993Ra01 | 组织蛋白酶G(CTSG)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD993Ra01 | 组织蛋白酶G(CTSG)多克隆抗体 | WB; IHC; ICC; IP. |
| MAD993Ra21 | 组织蛋白酶G(CTSG)单克隆抗体 | WB; IHC; ICC; IP. |
| SED993Ra | 组织蛋白酶G(CTSG)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMD993Ra | 组织蛋白酶G(CTSG)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| journal of maternal-fetal & neonatal medicine | Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes.[pubmed:28152632] |
| Lung | Mass Spectrometry Analysis of the Exhaled Breath Condensate and Proposal of Dermcidin and S100A9 as Possible Markers for Lung Cancer Prognosis[Pubmed: 31115649] |