武汉云克隆科技股份有限公司

粒细胞巨噬细胞集落刺激因子(GM-CSF)活性蛋白

Active Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF)

CSF2; GMCSF; Sargramostim; Molgramostin; Granulocyte-Macrophage Colony Stimulating Factor

  • 粒细胞巨噬细胞集落刺激因子(GM-CSF)活性蛋白产品包装(模拟)
  • 粒细胞巨噬细胞集落刺激因子(GM-CSF)活性蛋白产品包装(模拟)
  • APA045Mu01.jpgSDS-PAGE图
  • 粒细胞巨噬细胞集落刺激因子(GM-CSF)活性蛋白Figure . Western Blot; Sample: APA045Mu01; Antibody:PAA045Mu01
  • Certificate通过ISO 9001、ISO 13485质量体系认证

活性实验

Measured in a cell proliferation assay using mouse BMDC (bone marrow derived dendritic cells). The ED50 (median effective dose) for this effect is less than 0.25 ng/mL.
In-house data of APA045Mu01 used in cellular experiment:
Six-eight weeks old Balb/c mice were used for BMDC. At first, mouse femur and tibia were taken out, and then bone marrow was washed out with serum-free RPMI 1640 medium, followed by centrifugation at 1200RPM for 5min (4oC). ACK buffer was added to get rid of red blood cells, and then, centrifuged at 1200RPM for 5min (4oC). Cell pellets were collected and re-suspended, the cells were cultured in DMEM medium supplemented with 10% FBS, or DMEM medium supplemented with 10% FBS and GMCSF (APA045Mu01) at 37oC with 5% CO2 in thermostatic incubator. Three days later, cells were observed by microscope, and the result is shown in Figure 1.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号适用物种：Mus musculus (Mouse，小鼠)应用(仅供研究使用，不用于临床诊断！)
EPA045Mu61粒细胞巨噬细胞集落刺激因子(GM-CSF)真核蛋白Positive Control; Immunogen; SDS-PAGE; WB.
RPA045Mu02粒细胞巨噬细胞集落刺激因子(GM-CSF)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA045Mu01粒细胞巨噬细胞集落刺激因子(GM-CSF)活性蛋白Cell culture; Activity Assays.
APA045Mu61粒细胞巨噬细胞集落刺激因子(GM-CSF)活性蛋白Cell culture; Activity Assays.
RPA045Mu01粒细胞巨噬细胞集落刺激因子(GM-CSF)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA045Mu01粒细胞巨噬细胞集落刺激因子(GM-CSF)多克隆抗体WB; IHC
PAA045Mu06粒细胞巨噬细胞集落刺激因子(GM-CSF)多克隆抗体WB; IHC; ICC; IP.
PAA045Mu02粒细胞巨噬细胞集落刺激因子(GM-CSF)多克隆抗体WB; IHC; ICC; IP.
SEA045Mu粒细胞巨噬细胞集落刺激因子(GM-CSF)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
MEA045Mu粒细胞巨噬细胞集落刺激因子(GM-CSF)检测试剂盒(酶联免疫吸附试验法,小样本)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA045Mu粒细胞巨噬细胞集落刺激因子(GM-CSF)检测试剂盒(化学发光免疫分析法)Chemiluminescent immunoassay for Antigen Detection.
LMA045Mu粒细胞巨噬细胞集落刺激因子(GM-CSF)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSA045Mu01粒细胞巨噬细胞集落刺激因子(GM-CSF)检测试剂盒DIY材料(酶联免疫吸附试验法)Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志参考文献
Journal of Toxicology and Environmental HealthEffects of 900-MHz Microwave Radiation on γ-Ray-Induced Damage to Mouse Hematopoietic System[PubMed: 20391130]
5Safety and efficacy of the peptide-based therapeutic vaccine for HIV-1, Vacc-4×: a phase 2 randomised, double-blind, placebo-controlled trial[Pubmed:24525316]
Archives of VirologyGenetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central China[Pubmed:29305646]
BioMed Research InternationalActivated Macrophages of Monocytic Origin Predominantly Express Proinflammatory Cytokine Genes, Whereas Kupffer Cells Predominantly Express Anti …[]
bulletin of experimental biology and medicineQuantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin[Pubmed: 31183654]
Exp Mol PatholThe regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡­[33285209]
VaccineCo-delivery of PSMA antigen epitope and mGM-CSF with a cholera toxin-like chimeric protein suppressed prostate tumor growth via activating dendritic cells and?¡­[33612342]