成纤维细胞生长因子15(FGF15)活性蛋白

Active Fibroblast Growth Factor 15 (FGF15)

活性实验

Fibroblast growth factor 15 is a protein in mouse encoded by the Fgf15 gene. It is a member of the fibroblast growth factor (FGF) family but, like FGF19, FGF21 and FGF23, has endocrine functions. FGF15 subsequently acts on a cell surface receptor complex in hepatocytes to repress bile acid synthesis and gluconeogenesis, and to stimulate glycogen and protein synthesis.Besides, Fibroblast Growth Factor Receptor 4 (FGFR4) has been identified as an interactor of FGF15, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat FGF15 and recombinant rat FGFR4. Briefly, FGF15 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl then transferred to FGFR4-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF15 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of FGF15 and FGFR4 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号适用物种:Rattus norvegicus (Rat,大鼠)应用(仅供研究使用,不用于临床诊断!)
APL154Ra01成纤维细胞生长因子15(FGF15)活性蛋白Cell culture; Activity Assays.
RPL154Ra01成纤维细胞生长因子15(FGF15)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAL154Ra01成纤维细胞生长因子15(FGF15)多克隆抗体WB; IHC
MAL154Ra22成纤维细胞生长因子15(FGF15)单克隆抗体WB; IHC; ICC; IP.
MAL154Ra23成纤维细胞生长因子15(FGF15)单克隆抗体WB; IHC; ICC; IP.
MAL154Ra24成纤维细胞生长因子15(FGF15)单克隆抗体WB; IHC; ICC; IP.
MAL154Ra25成纤维细胞生长因子15(FGF15)单克隆抗体WB; IHC; ICC; IP.
MAL154Ra27成纤维细胞生长因子15(FGF15)单克隆抗体WB; IHC; ICC; IP.
MAL154Ra21成纤维细胞生长因子15(FGF15)单克隆抗体WB; IHC; ICC; IP.
WEL154Ra成纤维细胞生长因子15(FGF15)检测试剂盒(酶联免疫吸附试验法,宽范围)Enzyme-linked immunosorbent assay for Antigen Detection.
SEL154Ra成纤维细胞生长因子15(FGF15)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
CEL154Ra成纤维细胞生长因子15(FGF15)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LML154Ra成纤维细胞生长因子15(FGF15)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.
KSL154Ra01成纤维细胞生长因子15(FGF15)检测试剂盒DIY材料(酶联免疫吸附试验法)自己动手制作ELISA试剂盒的主要材料

参考文献

杂志参考文献
Endocrinology. Naringenin Prevents Obesity, Hepatic Steatosis, and Glucose Intolerance in Male Mice Independent of Fibroblast Growth Factor 21[PubMed: 25774553]
Inflammatory Bowel DiseasesAlterations in Enterohepatic Fgf15 Signaling and Changes in Bile Acid Composition Depend on Localization of Murine Intestinal Inflammation[pubmed:27580383]
J Lipid Res.Commensal bacteria at the crossroad between cholesterol homeostasis and chronic inflammation in atherosclerosis.[pubmed:28130274]
BMC Endocrine DisordersThe relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of glucose intolerance[10.1186/s12902-017-0211-5]
Journal of Physiology and BiochemistryThe ileal FGF15/19 to hepatic FGFR4 axis regulates liver regeneration after partial hepatectomy in mice[Pubmed:29468415]
Developmental CellFGF15 Activates Hippo Signaling to Suppress Bile Acid Metabolism and Liver Tumorigenesis[Pubmed: 30745141]
EndocrinologyInteractions between the gravitostat and the fibroblast growth factor system for the regulation of body weight[]
Food & FunctionLactoferrin promotes bile acid metabolism and reduces hepatic cholesterol deposition by inhibiting the farnesoid X receptor (FXR)-mediated enterohepatic axis[Pubmed: 31626262]
Journal of Translational MedicineGut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activation[Pubmed: 33036625]
Chin MedSi-Wu-Tang ameliorates fibrotic liver injury via modulating intestinal microbiota and bile acid homeostasis[34736501]
Int J Biol SciDynamics of the gut-liver axis in rats with varying fibrosis severity[Pubmed:35637968]