干扰素γ(IFNg)活性蛋白
Active Interferon Gamma (IFNg)
IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
活性实验
Interferon gamma (IFNγ) is a dimerized soluble cytokine that is the only member of the type II class of interferons. The importance of IFNγ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. It has been reported that IFN-γ promotes production of inducible Nitric Oxide Synthase (iNOS) in macrophages as an important activator. After stimulated with IFN-γ, morphological changes will occur in murine macrophage cell line (Raw 264.7 cells), and inducible nitric-oxide synthase (iNOS) in the cells will increase. Raw 264.7 cells were incubated in DMEM with IFN-γ (10ng/mL) for 24h, then cells were observed by inverted microscope and iNOS in cell lysates was detected by ELISA.
Effect of IFN-γ on morphological change of Raw 246.7 cells was shown in Figure 1.
Effect of IFN-γ on the expression of iNOS was shown in Table 1.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Cavia (Guinea pig ，豚鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| APA049Gu01 | 干扰素γ(IFNg)活性蛋白 | Cell culture; Activity Assays. |
| RPA049Gu01 | 干扰素γ(IFNg)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA049Gu01 | 干扰素γ(IFNg)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA049Gu91 | 干扰素γ(IFNg)多克隆抗体(辣根过氧化物酶标记) | WB; IHC; ICC. |
| LAA049Gu71 | 干扰素γ(IFNg)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA049Gu21 | 干扰素γ(IFNg)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA049Gu | 干扰素γ(IFNg)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA049Gu | 干扰素γ(IFNg)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA049Gu | 干扰素γ(IFNg)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
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| Environmental Research | In-vitro oxidative potential and inflammatory response of ambient PM2. 5 in a rural region of Northwest China: Association with chemical compositions and …[34863982] |
| Eur J Pharm Sci | Preclinical study of methotrexate-based hydrogels versus surfactant based liquid crystal systems on psoriasis treatment[34314841] |
| J Inflamm Res | Clenbuterol, a Selective β2-Adrenergic Receptor Agonist, Inhibits or Limits Post-Stroke Pneumonia, but Increases Infarct Volume in MCAO Mice[Pubmed:35058704] |
| Poult Sci | Salmonella phage CKT1 significantly relieves the body weight loss of chicks by normalizing the abnormal intestinal microbiome caused by hypervirulent …[Pubmed:35063807] |
| ACS Appl Mater Interfaces | Combinational Chemoimmunotherapy for Breast Cancer by Codelivery of Doxorubicin and PD-L1 siRNA Using a PAMAM-Incorporated Liposomal Nanoplatform[Pubmed:35138103] |
| Theranostics | Combating multidrug resistance and metastasis of breast cancer by endoplasmic reticulum stress and cell-nucleus penetration enhanced …[Pubmed:35401832] |
| Inflammopharmacology | Silymarin constrains diacetyl-prompted oxidative stress and neuroinflammation in rats: involvements of Dyn/GDNF and MAPK signaling pathway[Pubmed:35366745] |
| Journal of Nanobiotechnology | Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM[Pubmed:35351131] |
| Infection and Drug Resistance | Sweet's Syndrome Accompanied by Coinfection with Multiple Pathogens and Disseminated Mycobacterium phlei Infection Presenting with Osteolytic …[Pubmed:35592103] |
| Sci Rep | Clinical findings and predictive factors for positive anti-interferon-γ autoantibodies in patients suffering from a non-tuberculosis mycobacteria or Talaromyces …[Pubmed:35641599] |
| Biomedicines | Deletion of Toxoplasma Rhoptry Protein 38 (PruΔrop38) as a Vaccine Candidate for Toxoplasmosis in a Murine Model[Pubmed:35740356] |
| Viruses | Synergistic Pathogenicity by Coinfection and Sequential Infection with NADC30-like PRRSV and PCV2 in Post-Weaned Pigs[Pubmed:35215787] |
| Antioxidants | Blue Light Alters the Composition of the Jejunal Microbiota and Promotes the Development of the Small Intestine by Reducing Oxidative Stress[Pubmed:35204158] |
| Oxidative Medicine and Cellular Longevity | The Role of Aeromonas-Goblet Cell Interactions in Melatonin-Mediated Improvements in Sleep Deprivation-Induced Colitis[Pubmed:35355860] |
| Journal of Integrative Medicine | Salvadora persica extract attenuates cyclophosphamide-induced hepatorenal damage by modulating oxidative stress, inflammation and apoptosis in rats[Pubmed:35643766] |
| researchsquare | MSC-EVs: A Promising Modulator for Immune Response in Systemic Lupus Erythematosus[] |
| Mljekarstvo | Th1/Th2 cytokine polarization in milk according to different pathogens causing subclinical mastitis in cows[] |