白介素1家族成员9(IL1F9)活性蛋白
Active Interleukin 1 Family, Member 9 (IL1F9)
IL-1F9; IL36g; IL-1H1; IL-1RP2; IL1E; IL1H1; IL1RP2; Interleukin 36, Gamma; Interleukin-1 Homolog 1; Interleukin-1 epsilon; Interleukin 1-Related Protein 2
活性实验
IL36G (Interleukin 36 gamma), also known as IL-1F9 and IL-1H1 is a member of the IL-1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1F9 can induced IL-8 production in HacaT cells. To test the bioactivity of IL-1F9, HacaT cells were seeded into 24-well plate at a density of 1x105 cells/mL , and allowed to attach overnight before treated with certain concentrations of IL-1F9 for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of HacaT cells increased significantly after stimulated with IL-1F9 have shown in Figure1.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPL621Ra01 | 白介素1家族成员9(IL1F9)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APL621Ra01 | 白介素1家族成员9(IL1F9)活性蛋白 | Cell culture; Activity Assays. |
| PAL621Ra01 | 白介素1家族成员9(IL1F9)多克隆抗体 | WB; IHC; ICC; IP. |
参考文献
| 杂志 | 参考文献 |
| PLOS ONE | Interleukin (IL)-1β Is a Strong Inducer of IL-36γ Expression in Human Colonic Myofibroblasts[PubMed: 26562662] |
| Journal of Investigative Dermatology | Increased interleukin‐36γ expression in skin and sera of patients with atopic dermatitis and mycosis fungoides/Sézary syndrome[Pubmed:29314201] |
| Journal of Investigative Dermatology | For Review Only[] |
| international immunopharmacology | Acitretin inhibits IL-17A-induced IL-36 expression in keratinocytes by down-regulating IκBζ[Pubmed: 31863918] |
| J Agric Food Chem | Inhibition of HMGB1/TLR4 Signaling Pathway by Digitoflavone: A Potential Therapeutic Role in Alcohol-Associated Liver Disease[Pubmed:35212223] |
| Br J Pharmacol | Modulation of IL‐36‐based inflammatory feedback loop through hepatocytes‐derived IL‐36R‐P2X7R axis improves steatosis in alcoholic steatohepatitis[Pubmed:35481896] |