白介素1受体拮抗剂(IL1RA)活性蛋白

Active Interleukin 1 Receptor Antagonist (IL1RA)

IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP;IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra

活性实验

The interleukin-1 receptor antagonist (IL-1RA) is a member of the interleukin 1 cytokine family. IL1Ra is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL1β. IL-1RN is an agent that binds to the cell surface interleukin-1 receptor (IL-1R), which would prevent IL-1 from intracellular signal transduction. Thus, a binding ELISA assay was conducted to detect the interaction of recombinant simian IL-1RN and recombinant human IL-1R1. Briefly, IL-1RA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to IL-1R1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL-1RN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of IL-1RA and IL-1R1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号适用物种:Rhesus monkey (Simian,恒河猴)应用(仅供研究使用,不用于临床诊断!)
RPA223Si01白介素1受体拮抗剂(IL1RA)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
APA223Si01白介素1受体拮抗剂(IL1RA)活性蛋白Cell culture; Activity Assays.
PAA223Si01白介素1受体拮抗剂(IL1RA)多克隆抗体WB; IHC; ICC; IP.
LAA223Si71白介素1受体拮抗剂(IL1RA)多克隆抗体(生物素标记)WB; IHC; ICC.
LAA223Si81白介素1受体拮抗剂(IL1RA)多克隆抗体(异硫氰酸荧光素标记)WB; IHC; ICC; IF.
MAA223Si21白介素1受体拮抗剂(IL1RA)单克隆抗体WB; IHC; ICC; IP.
SEA223Si白介素1受体拮抗剂(IL1RA)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA223Si白介素1受体拮抗剂(IL1RA)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.

参考文献

杂志参考文献
The Veterinary JournalMucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel disease[ScienceDirect: S1090023312000962]
Daily quercetin supplementation over a period of two weeks results in a moderate accumulation of total plasma flavonols in horses[Researchgate:Source]
Journal of Equine Veterinary ScienceSynovial Fluid and Serum Concentrations of Interleukin-1 Receptor Antagonist and Interleukin-1[Science: Article]
Journal of Equine Veterinary ScienceA Two-Week Quercetin Supplementation in Horses Results in Moderate Accumulation of Plasma Flavonol Concentrations[Science: Article]
Scientific publications in peer reviewed journalsEvidence-based review of efficacy and adverse effects of joint medication and evaluation of synovial fluid and serum markers for osteoarthritis in the horse[receive:101658]
Multiple Sclerosis and Related DisordersInterleukin 1 receptor antagonist and 2′-5′-oligoadenylate synthetase-like molecules as novel biomarkers for multiple sclerosis patients in Bahrain[pubmed:29141788]
Advances in Medical SciencesA similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics[pubmed:28822267]
Archives of Medical ResearchWinter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young …[Pubmed:29379533]
Journal of Veterinary Internal MedicineEffect of interleukin‐1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease[Pubmed:29572935]
Frontiers in PharmacologyM10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in Mice[Pubmed: 33041798]