武汉云克隆科技股份有限公司

Osteopontin (OPN), also known as secreted phosphoprotein 1 (SPP1), is a highly conserved, multifunctional glycoprotein that plays pivotal roles in various physiological and pathological processes. Encoded by the SPP1 gene, this protein is characterized by its arginine-glycine-aspartic acid (RGD) motif, which enables it to interact with integrin receptors, facilitating cell adhesion, migration, and signaling. As OPN has the function of cell adhesion, we measure the activity of recombinant bovine OPN by the ability of the immobilized protein to support the adhesion of 293T cells. When cells are added to different concentrations of recombinant bovine OPN coated plates, cells will adhere after 2 hour incubation at 37 ℃. The adhesion of 293T after 2 hour incubation at 37 ℃ observed by inverted microscope was shown in Figure 1. Cells adherent was in a dose dependent manner, the result was shown in Figure 2, the EC50 was 0.27 μg/ml.