骨桥素(OPN)活性蛋白
Active Osteopontin (OPN)
SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin
- 编号APA899Hu61
- 物种Homo sapiens (Human，人)相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 95%
- 等电点4.3
- 应用Cell culture; Activity Assays.
- 下载英文说明书 中文说明书
- 规格10µg50µg200µg1mg5mg
- 价格¥ 566¥ 1416¥ 2832¥ 8496¥ 21240
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产品包装(模拟)
产品包装(模拟)
Gene sequencing
SDS-PAGE图
Figure. Western Blot; Sample: Recombinant OPN, Human.
通过ISO 9001、ISO 13485质量体系认证
活性实验
Osteopontin (OPN), a multifunctional phosphorylated glycoprotein, plays an important role in neutrophil recruitment and was found to induce the expression of proinflammatory chemokines including MCP-1 and MIP-1β which would promote migration and recruitment of inflammatory cells. It has been reported that OPN induces MCP-1 expression through the NF-kappa B pathways in MCF-7 breast cancer cell line. Briefly, MCF-7 cells were seeded overnight at a density of 1x105 cells/mL, and treated with or without OPN(100ng/ml、200ng/ml) for 24h and MCP-1 levels in the cell supernatant were determined by ELISA.
MCP-1 levels in the cell supernatant of MCF-7 cells increased significantly after stimulated with OPN, the data was shown in Table 1 and Figure 1.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| EPA899Hu61 | 骨桥素(OPN)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA899Hu02 | 骨桥素(OPN)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA899Hu62 | 骨桥素(OPN)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA899Hu61 | 骨桥素(OPN)活性蛋白 | Cell culture; Activity Assays. |
| PAA899Hu06 | 骨桥素(OPN)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA899Hu02 | 骨桥素(OPN)多克隆抗体 | WB; IHC; ICC; IP; FCM |
| LAA899Hu71 | 骨桥素(OPN)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA899Hu22 | 骨桥素(OPN)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA899Hu23 | 骨桥素(OPN)单克隆抗体 | WB |
| MAA899Hu24 | 骨桥素(OPN)单克隆抗体 | WB |
| MAA899Hu25 | 骨桥素(OPN)单克隆抗体 | WB |
| MAA899Hu21 | 骨桥素(OPN)单克隆抗体 | WB |
| SEA899Hu | 骨桥素(OPN)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEA899Hu | 抗骨桥素抗体(Anti-OPN)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCA899Hu | 骨桥素(OPN)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA899Hu | 骨桥素(OPN)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA899Hu01 | 骨桥素(OPN)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Journal of Biomedical Materials Research Part A | Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture[PubMed: 19280635] |
| Osteoarthritis and Cartilage | Elevated osteopontin level of synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis patients[PubMed: 19747583] |
| The Journal of Rheumatology | Thrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis.[Jrheum: source] |
| International Journal of Pharmaceutics | Suppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester)[ScienceDirect: S0378517312003742] |
| European Cells and matreials | The use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineering[Ecmjournal: Source] |
| Journal of Equine Veterinary Science | Influence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse[ScienceDirect: Source] |
| Basic & Clinical Pharmacology & Toxicology | Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions[Wiley:Source] |
| Journal of Cellular Biochemistry | Tension Force-Induced ATP Promotes Osteogenesis Through P2X7 Receptor in Osteoblasts[Pubmed:24905552] |
| Naunyn-Schmiedeberg's Archives of Pharmacology | Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats[Pubmed:25038619] |
| J Endocrinol Invest.? | The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders[Pubmed:25245337] |
| FEBS Letters | Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells[Pubmed:25542352] |
| Gene | Effects of magnesium degradation products on mesenchymal stem cell fate and osteoblastogenesis[PubMed: 26283150] |
| Medicine (Baltimore) | An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer[PubMed: 26266370] |
| Journal of Biomaterials and Tissue Engineering | Osteogenic Differentiation of Canine Adipose Stem Cells Cultured in Alginate-Fibrin-Based Hydrogel[Content: Asp] |
| Primasari | Osteopontin level correlates negatively with tumor shrinkage in neoadjuvant chemoradiation of locally advanced rectal cancer[Index.Php: Amor] |
| Int J Biol Sci | Defective Initiation of Liver Regeneration in Osteopontin-Deficient Mice after Partial Hepatectomy due to Insufficient Activation of IL-6/Stat3 Pathway[PubMed: 26327817] |
| Journal of Kunming Medical University / Kunming Yike Daxue Xuebao | Perioperative Clinical Investigation of Serum VEGF, CA19-9 and OPN Levels in Patients with Colorectal Carcinoma.[Ebscohost] |
| Am J Pathol. | Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy.[Pubmed:26963343] |
| circulation journal | Stenotic Bicuspid and Tricuspid Aortic Valves - Micro-Computed Tomography and Biological Indices of Calcification.[pubmed:28344201] |
| Journal of Cardiovascular Electrophysiology | Effect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure.[pubmed:28127817] |
| American Journal of Translational Research | Osteopontin stimulates matrix metalloproteinase expression through the nuclear factor-κB signaling pathway in rat temporomandibular joint and condylar chondrocytes.[pubmed:28337262] |
| Diabetes Care | Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans[pubmed:29212824] |
| Biomedicine & pharmacotherapy | Icaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation.[pubmed:28742995] |
| Journal of Alzheimers Disease | Urine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods[Pubmed:30040720] |
| Indian Journal of Nephrology | Osteopontin, cardiovascular risk factors and carotid intima-Media thickness in chronic kidney disease[id:233806] |
| Carbon | In Situ Self-Assembly of Graphene Oxide/Polydopamine/Sr2+ Nanosheets on Titanium Surfaces for Enhanced Osteogenic Differentiation of Mesenchymal Stem Cells[Doi: 10.1016/j.carbon.2018.10.081] |
| current eye research | Detection of proteins associated with extracellular matrix regulation in the aqueous humour of patients with primary glaucoma.[Pubmed: 30994369] |
| Journal of Proteomics | Identification of urinary candidate biomarkers of cisplatin-induced nephrotoxicity in patients with carcinoma[Pubmed: 31629959] |
| Acta Biomaterialia | Inflammatory response to magnesium-based biodegradable implant materials[Pubmed: 31610341] |
| Journal of Clinical Medicine | Calcium Kidney Stones are Associated with Increased Risk of Carotid Atherosclerosis: the Link between Urinary Stone Risks, Carotid Intima-Media Thickness, and …[Pubmed: 32182704] |
| EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES | Febuxostat attenuates vascular calcification induced by vitamin D3 plus nicotine in rats[Pubmed: 33010420] |
| [ENGINEERING OF BIOMATERIALS | Osteoconductive potential of PLGA/bioglass composite biomaterials[] |
| Biomolecules | Structural Constraint of Osteopontin Facilitates Efficient Binding to CD44[34070790] |
| Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells[] | |
| Parasit Vectors | Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate …[34857049] |
| Medicina (Kaunas) | Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study[Pubmed:35743980] |