武汉云克隆科技股份有限公司

Taxilin Alpha (TXLNa) also known as interleukin-14 (IL-14) is a cytokine that controls the growth and proliferation of both normal and cancerous B cells. TXLNa induces B-cell proliferation, inhibits antibody secretion, and expands selected B-cell subgroups. This interleukin is produced mainly by T cells and certain malignant B cells. Besides, Phosphodiesterase 4D Interacting Protein (PDE4DIP) has been identified as an interactor of TXLNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse TXLNa and recombinant human PDE4DIP. Briefly, TXLNa were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100ul were then transferred to PDE4DIP-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TXLNa pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TXLNa and PDE4DIP was shown in Figure 1, the EC50 for this effect is 0.09ug/mL.