肿瘤坏死因子β(TNFb)活性蛋白

Active Tumor Necrosis Factor Beta (TNFb)

LT; LTA; TNF-B; TNFSF1; Tumor Necrosis Factor Ligand Superfamily Member 1; Lymphotoxin Alpha

活性实验

TNF-β, a member of the tumor necrosis factor family, is a potent lymphoid factor that exerts cytotoxic effects on a wide range of tumor cells. The biological effects of TNF-β are very similar to TNF-α, due to the similarity of molecular structure and the receptors. As reported, TNF-β could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNF-β (10ng/mL) for 8h, 24h, 48h, 72h, then cells were observed by inverted microscope and IL-1β was detected in the cell supernatant by ELISA .
Cell necrosis after incubation with TNF-β(10ng/mL) for 72h was shown in Figure 1.

The production of IL-1β after incubation with TNF-β (10ng/mL) for 8h is shown in Table 1.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号适用物种:Homo sapiens (Human,人)应用(仅供研究使用,不用于临床诊断!)
APA134Hu01肿瘤坏死因子β(TNFb)活性蛋白Cell culture; Activity Assays.
RPA134Hu01肿瘤坏死因子β(TNFb)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA134Hu01肿瘤坏死因子β(TNFb)多克隆抗体WB; IHC; ICC; IP.
MAA134Hu22肿瘤坏死因子β(TNFb)单克隆抗体WB; IHC; ICC; IP.
MAA134Hu21肿瘤坏死因子β(TNFb)单克隆抗体WB; IHC; ICC; IP.
SEA134Hu肿瘤坏死因子β(TNFb)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA134Hu肿瘤坏死因子β(TNFb)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.

参考文献

杂志参考文献
Journal of Applied PhysiologySimvastatin attenuates sympathetic hyperinnervation to prevent atrial fibrillation during the postmyocardial infarction remodeling process[PubMed: 22984252]
Journal of Translational MedicineEffectiveness of pure argon for renal transplant preservation in a preclinical pig model of heterotopic autotransplantation[Pubmed:26847569]
International Journal of Stem Cell Research and TransplantationProtective Role of Bone Marrow Derived Mesenchymal Stem Cells-Conditioned Medium in the Infarcted Myocardium: The Potential Role of Selected Cytokines[IJST-2328-3548-04-803]
Probiotics Antimicrob ProteinsEffect of Multi-Microbial Probiotic Formulation Bokashi on Pro-and Anti-Inflammatory Cytokines Profile in the Serum, Colostrum and Milk of Sows, and in a …[Pubmed:29305686]
Parasites & VectorsFasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism …[Pubmed: 32680553]
Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort[]
BMC MUSCULOSKELETAL DISORDERSElevated lymphotoxin-¦Á (TNF¦Â) is associated with intervertebral disc degeneration[33441130]