抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Monoclonal Antibody
G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH
- 编号CAB932Mi22
- 物种Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物无标记物
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度1mg/ml
- 且适物种Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Cavia (Guinea pig ，豚鼠), Hamster (Chinese hamster，仓鼠), Oryctolagus cuniculus (Rabbit，兔), Sus scrofa; Porcine (Pig，猪), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Ovis aries; Ovine (Sheep，绵羊), Equus caballus; Equine (Horse，马), Chicken (Gallus，鸡)
- 应用WB; IHC; ICC/IF
- 下载英文说明书 中文说明书
- 规格20µl100µl200µl1ml10ml
- 价格¥ 644¥ 1504¥ 2148¥ 5370¥ 21480
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产品包装(模拟)
产品包装(模拟)
DAB staining on IHC-P; Sample: Human Placenta Tissue; Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Rectum Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Lung Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
DAB staining on IHC-P;
Sample: Human Ovarian cancer Tissue;
Primary Ab: 5ug/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20μg/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
Western Blot; Sample: Lane1: Rat Cerebrum lysate; Lane2: Rat Heart lysate; Lane3: A549 cell lysate; Lane4: HepG2 cell lysate Primary Ab: 1µg/ml Mouse Anti-Multi-species GAPDH Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Human Placenta lysate; Lane3: Porcine Cerebrum lysate; Lane4: Bovine Cerebrum lysate; Lane5: Gallus Cerebrum lysate; Lane6: Equine Liver lysate; Lane7: Caprine Cerebrum lysate<br/>Primary Ab: 3µg/ml Mouse Anti-Multi-species GAPDH Antibody<br/>Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody<br/>(Catalog: SAA544Mu19)
Western Blot analysis of GAPDH in various cell lines
Lane1: DU145 whole cell lysate
Lane2: OS-RC-2 whole cell lysate
Lane3: T-47D whole cell lysate
Lane4: HEC-1B whole cell lysate
Lane5: HepG2 whole cell lysate
Lane6: HEK-293 whole cell lysate
Lysates/proteins at 20µg per lane.
Primary Ab: anti-GAPDH antibody (CAB932Mi22) at 0.1ng/ml
Secondary Ab: HRP-conjugated Rabbit anti-mouse antibody (SAA544Mu09) at 1/10000 dilution
Developed using the ECL technique.
Calculated MW: 36KD
Observed MW: 36KD
Exposure time: 1 min
Western Blot; Sample: Lane1: Human Lung lysate; Lane2: 293T cell lysate; Lane3: Cavia Liver lysate; Lane4: Cavia Kidney lysate; Lane5: Gallus Kidney lysate; Lane6: Bovine Liver lysate; Lane7: Bovine Kidney lysate; Lane8: Rabbit Kidney lysate; Lane9: Caprine Liver lysate; Lane10: Mouse Liver lysate; Lane11: Rat Liver lysate; Lane12: Rat Kidney lysate
Primary Ab: 0.5µg/ml Mouse Anti-Multi-species GAPDH Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对GAPDH的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别GAPDH。
用法
Enzyme-Linked Immune Sorbent Assay:
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| CAB932Mi01 | 抗甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体 | WB |
| CAB932Mi22 | 抗甘油醛-3-磷酸脱氢酶(GAPDH)单克隆抗体 | WB; IHC; ICC/IF |
参考文献
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