武汉云克隆科技股份有限公司

70kDa热休克蛋白1样蛋白(HSPA1L)多克隆抗体

Polyclonal Antibody to Heat Shock 70kDa Protein 1 Like Protein (HSPA1L)

HSP-A1L; HSPA1-L; HSP70-HOM; Hum70t

  • 70kDa热休克蛋白1样蛋白(HSPA1L)多克隆抗体产品包装(模拟)
  • 70kDa热休克蛋白1样蛋白(HSPA1L)多克隆抗体产品包装(模拟)
  • 70kDa热休克蛋白1样蛋白(HSPA1L)多克隆抗体IHC图
  • PAB062Hu02.jpgFigure. Western Blot; Sample: Recombinant HSPA1L, Human.
  • 70kDa热休克蛋白1样蛋白(HSPA1L)多克隆抗体Western Blot; Sample: Raji cell lysate; <br/>Primary Ab: 5µg/ml Rabbit Anti-Human HSPA1L Antibody<br/>Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
  • 70kDa热休克蛋白1样蛋白(HSPA1L)多克隆抗体Knockout Varification:
    Lane 1: Wild-type Raji cell lysate;
    Lane 2: HSPA1L knockout Raji cell lysate;
    Predicted MW: 70kd
    Observed MW: 70kd
    Primary Ab: 5µg/ml Rabbit Anti-Human HSPA1L Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • 70kDa热休克蛋白1样蛋白(HSPA1L)多克隆抗体Western Blot; Sample: Hela cell lysate; <br/>Primary Ab: 5µg/ml Rabbit Anti-Human HSPA1L Antibody<br/>Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
  • 70kDa热休克蛋白1样蛋白(HSPA1L)多克隆抗体Western Blot; Sample: HepG2 cell lysate; <br/>Primary Ab: 5µg/ml Rabbit Anti-Human HSPA1L Antibody<br/>Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
  • 70kDa热休克蛋白1样蛋白(HSPA1L)多克隆抗体Raji human Burkitt's lymphoma cell line was stained with rabbit Anti-human HSPA1L Polyclonal Antibody (Catalog # PAB062Hu02, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15).Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • 70kDa热休克蛋白1样蛋白(HSPA1L)多克隆抗体Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human HSPA1L Polyclonal Antibody (Catalog PAB062Hu02, filled red histogram) or Isotype control antibody (Catalog IS067-Mu11, filled green histogram).
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对HSPA1L的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别HSPA1L。

用法

Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:10μg/ml;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

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