PAA822Mu01 | 60kD热休克蛋白(Hsp60)多克隆抗体 | Mus musculus (Mouse，小鼠) USCN(武汉云克隆科技股份有限公司官方网站)

60kD热休克蛋白(Hsp60)多克隆抗体

DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse HSPD1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Cardiac Muscle Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse HSPD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Mouse Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Mouse HSPD1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse Hsp60 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse Hsp60 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Hela cell
Primary Ab: 10µg/ml Rabbit Anti-Mouse Hsp60 Antibody
Second Ab: 2μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Western Blot; Sample: Mouse Heart lysate Primary Ab: 0.2µg/ml Rabbit Anti-Mouse Hsp60 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 2µg/mL Rabbit Anti-Mouse HSPD1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 2µg/mL Rabbit Anti-Mouse HSPD1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2µg/mL Rabbit Anti-Mouse HSPD1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Western Blot; Samples: Lane1: NIH/3T3 cell lysate; Lane2: MCF7 cell lysate;
Primary Ab: 0.2µg/ml Rabbit Anti-Mouse Hsp60 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

该抗体是针对Hsp60的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别Hsp60。

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

杂志	参考文献
Sci Rep	Hsp60 response in experimental and human temporal lobe epilepsy[PubMed: 25801186]
Cellular and Molecular Gastroenterology and Hepatology	Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitro[Pubmed: 30878663]
Breast Cancer Res Treat	Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer[Pubmed: 32601970]

编号	适用物种：Mus musculus (Mouse，小鼠)	应用(仅供研究使用，不用于临床诊断！)
RPA822Mu01	60kD热休克蛋白(Hsp60)重组蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
RPA822Mu02	60kD热休克蛋白(Hsp60)重组蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAA822Mu01	60kD热休克蛋白(Hsp60)多克隆抗体	WB; IHC; ICC/IF
PAA822Mu02	60kD热休克蛋白(Hsp60)多克隆抗体	WB; IHC; ICC/IF
SEA822Mu	60kD热休克蛋白(Hsp60)检测试剂盒(酶联免疫吸附试验法)	Enzyme-linked immunosorbent assay for Antigen Detection.
SCA822Mu	60kD热休克蛋白(Hsp60)检测试剂盒(化学发光免疫分析法)	Chemiluminescent immunoassay for Antigen Detection.
LMA822Mu	60kD热休克蛋白(Hsp60)等多因子检测试剂盒(流式荧光发光法)	FLIA Kit for Antigen Detection.
KSA822Mu01	60kD热休克蛋白(Hsp60)检测试剂盒DIY材料(酶联免疫吸附试验法)	Main materials for "Do It (ELISA Kit) Yourself".