PAD559Hu01 | 丝裂原激活蛋白激酶激酶1(MAP2K1)多克隆抗体 | Homo sapiens (Human，人) USCN(武汉云克隆科技股份有限公司官方网站)

丝裂原激活蛋白激酶激酶1(MAP2K1)多克隆抗体

编号PAD559Hu01
物种Homo sapiens (Human，人)相同的名称，不同的物种。
来源多抗制备
宿主兔
效价-
Ig类型IgG
纯化方式抗原特异性亲和纯化
标记物无标记物
免疫原RPD559Hu01-丝裂原激活蛋白激酶激酶1(MAP2K1)重组蛋白
缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
性状液体
浓度500µg/mL
且适物种Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，猪)
应用WB; IHC; ICC; IP.
如果抗体需用于流式细胞术，请参见流式抗体。
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DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human MAP2K1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human MAP2K1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human MAP2K1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Thyroid cancer Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human MAP2K1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
DAB staining on IHC-P;
Sample: Human Lung cancer Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human MAP2K1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
FITC staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Human MAP2K1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)

Figure. Western Blot; Sample: Recombinant MAP2K1, Human.
Western Blot; Sample: Lane1: Porcine Cerebrum lysate; Lane2: Mouse Cerebrum lysate; Lane3: Rat Cerebrum lysate; Lane4: U937 cell lysate; Lane5: U87MG cell lysate; Lane6: HepG2 cell lysate
Primary Ab: 1µg/ml Rabbit Anti-Human MAP2K1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

该抗体是针对MAP2K1的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别MAP2K1。

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

杂志	参考文献
Graefe's Archive for Clinical and Experimental Ophthalmology	Role of RhoA/MERK1/ERK1/2/iNOS signaling in ocular ischemic syndrome[pubmed:27538909]
PLOS ONE	Identification of Biomarkers of Impaired Sensory Profiles among Autistic Patients.[pubmed:27824861]
Cancers	Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid Leukemia[Pubmed: 31718075]
Cancers (Basel)	BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 581[33540760]

编号	适用物种：Homo sapiens (Human，人)	应用(仅供研究使用，不用于临床诊断！)
RPD559Hu01	丝裂原激活蛋白激酶激酶1(MAP2K1)重组蛋白	Positive Control; Immunogen; SDS-PAGE; WB.
PAD559Hu01	丝裂原激活蛋白激酶激酶1(MAP2K1)多克隆抗体	WB; IHC; ICC; IP.
MAD559Hu21	丝裂原激活蛋白激酶激酶1(MAP2K1)单克隆抗体	WB; IHC; ICC; IP.
SED559Hu	丝裂原激活蛋白激酶激酶1(MAP2K1)检测试剂盒(酶联免疫吸附试验法)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMD559Hu	丝裂原激活蛋白激酶激酶1(MAP2K1)等多因子检测试剂盒(流式荧光发光法)	FLIA Kit for Antigen Detection.