武汉云克隆科技股份有限公司

骨桥素(OPN)多克隆抗体

Polyclonal Antibody to Osteopontin (OPN)

SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin

  • 骨桥素(OPN)多克隆抗体产品包装(模拟)
  • 骨桥素(OPN)多克隆抗体产品包装(模拟)
  • 骨桥素(OPN)多克隆抗体DAB staining on IHC-P; Samples: Porcine Kidney Tissue; Primary Ab: 30µg/ml Rabbit Anti-Porcine OPN Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • 骨桥素(OPN)多克隆抗体DAB staining on IHC-P;
    Sample: Porcine Kidney Tissue
    Primary Ab: 30µg/ml Rabbit Anti-Porcine OPN Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA899Po01.jpgFigure. Western Blot; Sample: Recombinant OPN, porcine.
  • 骨桥素(OPN)多克隆抗体Western Blot; Sample: Lane1: Mouse Kidney lysate; Lane2: Human Jurkat cell lysate; Lane3: Human Urine; Lane4: Porcine Kidney lysate;
    Primary Ab: 1µg/mL Rabbit Anti-Porcine OPN Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

该抗体是针对OPN的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别OPN。

用法

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

储存

经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

赠品

相关产品

编号适用物种：Sus scrofa; Porcine (Pig，猪)应用(仅供研究使用，不用于临床诊断！)
RPA899Po01骨桥素(OPN)重组蛋白Positive Control; Immunogen; SDS-PAGE; WB.
PAA899Po01骨桥素(OPN)多克隆抗体WB; IHC; ICC; IP.
MAA899Po21骨桥素(OPN)单克隆抗体WB; IHC; ICC; IP.
MAA899Po22骨桥素(OPN)单克隆抗体WB; IHC; ICC; IP.
MAA899Po23骨桥素(OPN)单克隆抗体WB; IHC; ICC; IP.
MAA899Po24骨桥素(OPN)单克隆抗体WB; IHC; ICC; IP.
SEA899Po骨桥素(OPN)检测试剂盒(酶联免疫吸附试验法)Enzyme-linked immunosorbent assay for Antigen Detection.
LMA899Po骨桥素(OPN)等多因子检测试剂盒(流式荧光发光法)FLIA Kit for Antigen Detection.

参考文献

杂志参考文献
Journal of Biomedical Materials Research Part AProliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture[PubMed: 19280635]
Osteoarthritis and CartilageElevated osteopontin level of synovial fluid and articular cartilage is associated with disease severity in knee osteoarthritis patients[PubMed: 19747583]
The Journal of RheumatologyThrombin-cleaved osteopontin levels in synovial fluid correlate with disease severity of knee osteoarthritis.[Jrheum: source]
International Journal of PharmaceuticsSuppression of tumor growth in xenograft model mice by small interfering RNA targeting osteopontin delivery using biocompatible poly(amino ester)[ScienceDirect: S0378517312003742]
European Cells and matreialsThe use of non-viral gene vectors for bioactive poly-(D, L-lactide) implant surfaces in bone tissue engineering[Ecmjournal: Source]
Journal of Equine Veterinary ScienceInfluence of Seminal Plasma Antioxidants and Osteopontin on Fertility of the Arabian Horse[ScienceDirect: Source]
Basic & Clinical Pharmacology & ToxicologyTreatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions[Wiley:Source]
Journal of Cellular BiochemistryTension Force-Induced ATP Promotes Osteogenesis Through P2X7 Receptor in Osteoblasts[Pubmed:24905552]
Naunyn-Schmiedeberg's Archives of PharmacologyTreatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats[Pubmed:25038619]
J Endocrinol Invest.?The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders[Pubmed:25245337]
FEBS LettersLow-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells[Pubmed:25542352]
GeneEffects of magnesium degradation products on mesenchymal stem cell fate and osteoblastogenesis[PubMed: 26283150]
Medicine (Baltimore)An Attempt to Evaluate Selected Aspects of “Bone–Fat Axis” Function in Healthy Individuals and Patients With Pancreatic Cancer[PubMed: 26266370]
Journal of Biomaterials and Tissue EngineeringOsteogenic Differentiation of Canine Adipose Stem Cells Cultured in Alginate-Fibrin-Based Hydrogel[Content: Asp]
PrimasariOsteopontin level correlates negatively with tumor shrinkage in neoadjuvant chemoradiation of locally advanced rectal cancer[Index.Php: Amor]
Int J Biol SciDefective Initiation of Liver Regeneration in Osteopontin-Deficient Mice after Partial Hepatectomy due to Insufficient Activation of IL-6/Stat3 Pathway[PubMed: 26327817]
Journal of Kunming Medical University / Kunming Yike Daxue XuebaoPerioperative Clinical Investigation of Serum VEGF, CA19-9 and OPN Levels in Patients with Colorectal Carcinoma.[Ebscohost]
Am J Pathol. Serum Osteopontin as a Novel Biomarker for Muscle Regeneration in Duchenne Muscular Dystrophy.[Pubmed:26963343]
circulation journalStenotic Bicuspid and Tricuspid Aortic Valves　- Micro-Computed Tomography and Biological Indices of Calcification.[pubmed:28344201]
Journal of Cardiovascular ElectrophysiologyEffect of Cardiac Resynchronization Therapy on Myocardial Fibrosis and Relevant Cytokines in a Canine Model With Experimental Heart Failure.[pubmed:28127817]
American Journal of Translational ResearchOsteopontin stimulates matrix metalloproteinase expression through the nuclear factor-κB signaling pathway in rat temporomandibular joint and condylar chondrocytes.[pubmed:28337262]
Diabetes CareTargeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans[pubmed:29212824]
Biomedicine & pharmacotherapyIcaritin induces MC3T3-E1 subclone14 cell differentiation through estrogen receptor-mediated ERK1/2 and p38 signaling activation.[pubmed:28742995]
Journal of Alzheimers DiseaseUrine-Based Biomarkers for Alzheimer's Disease Identified Through Coupling Computational and Experimental Methods[Pubmed:30040720]
Indian Journal of NephrologyOsteopontin, cardiovascular risk factors and carotid intima-Media thickness in chronic kidney disease[id:233806]
CarbonIn Situ Self-Assembly of Graphene Oxide/Polydopamine/Sr2+ Nanosheets on Titanium Surfaces for Enhanced Osteogenic Differentiation of Mesenchymal Stem Cells[Doi: 10.1016/j.carbon.2018.10.081]
current eye researchDetection of proteins associated with extracellular matrix regulation in the aqueous humour of patients with primary glaucoma.[Pubmed: 30994369]
Journal of ProteomicsIdentification of urinary candidate biomarkers of cisplatin-induced nephrotoxicity in patients with carcinoma[Pubmed: 31629959]
Acta BiomaterialiaInflammatory response to magnesium-based biodegradable implant materials[Pubmed: 31610341]
Journal of Clinical MedicineCalcium Kidney Stones are Associated with Increased Risk of Carotid Atherosclerosis: the Link between Urinary Stone Risks, Carotid Intima-Media Thickness, and …[Pubmed: 32182704]
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCESFebuxostat attenuates vascular calcification induced by vitamin D3 plus nicotine in rats[Pubmed: 33010420]
[ENGINEERING OF BIOMATERIALSOsteoconductive potential of PLGA/bioglass composite biomaterials[]
BiomoleculesStructural Constraint of Osteopontin Facilitates Efficient Binding to CD44[34070790]
Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells[]
Parasit VectorsStructural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate …[34857049]
Medicina (Kaunas)Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study[Pubmed:35743980]