载脂蛋白B100(APOB100)检测试剂盒(化学发光免疫分析法)
CLIA Kit for Apolipoprotein B100 (APOB100)
Apo-B100
产品包装(模拟)
产品包装(模拟)
实验结果图
标准曲线图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测载脂蛋白B100(APOB100)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的载脂蛋白B100(APOB100)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 89-103 | 99 |
| EDTA plasma(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 80-103 | 85 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的载脂蛋白B100(APOB100)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中载脂蛋白B100(APOB100)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-98% | 90-97% | 84-102% | 95-102% |
| EDTA plasma(n=5) | 80-99% | 95-102% | 81-88% | 95-105% |
| heparin plasma(n=5) | 91-99% | 90-104% | 83-97% | 88-103% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100μL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分钟；
8. 读数。
实验原理
将载脂蛋白B100(APOB100)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的载脂蛋白B100(APOB100)与连接于固相载体上的抗体结合，然后加入生物素化的载脂蛋白B100(APOB100)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的载脂蛋白B100(APOB100)呈正相关。用化学发光仪测定相对光单位（RLU），计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| NPA603Hu01 | 载脂蛋白B100(APOB100)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA603Hu01 | 载脂蛋白B100(APOB100)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA603Hu01 | 载脂蛋白B100(APOB100)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA603Hu02 | 载脂蛋白B100(APOB100)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA603Hu71 | 载脂蛋白B100(APOB100)多克隆抗体(生物素标记) | IHC |
| MAA603Hu23 | 载脂蛋白B100(APOB100)单克隆抗体 | WB; IHC |
| MAA603Hu24 | 载脂蛋白B100(APOB100)单克隆抗体 | WB |
| MAA603Hu25 | 载脂蛋白B100(APOB100)单克隆抗体 | WB |
| MAA603Hu29 | 载脂蛋白B100(APOB100)单克隆抗体 | WB |
| MAA603Hu27 | 载脂蛋白B100(APOB100)单克隆抗体 | IHC |
| MAA603Hu21 | 载脂蛋白B100(APOB100)单克隆抗体 | WB; IHC |
| MAA603Hu22 | 载脂蛋白B100(APOB100)单克隆抗体 | WB; IHC |
| SEA603Hu | 载脂蛋白B100(APOB100)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA603Hu | 载脂蛋白B100(APOB100)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA603Hu | 载脂蛋白B100(APOB100)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
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