基质金属蛋白酶2(MMP2)检测试剂盒(化学发光免疫分析法)
CLIA Kit for Matrix Metalloproteinase 2 (MMP2)
CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase
产品包装(模拟)
产品包装(模拟)
实验结果图
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测基质金属蛋白酶2(MMP2)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的基质金属蛋白酶2(MMP2)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 99-105 | 102 |
| heparin plasma(n=5) | 96-104 | 101 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的基质金属蛋白酶2(MMP2)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中基质金属蛋白酶2(MMP2)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-101% | 79-88% | 88-98% | 79-103% |
| heparin plasma(n=5) | 88-102% | 83-93% | 82-99% | 83-93% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100μL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加底物100µL，37°C孵育10分钟；
8. 读数。
实验原理
将基质金属蛋白酶2(MMP2)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的基质金属蛋白酶2(MMP2)与连接于固相载体上的抗体结合，然后加入生物素化的基质金属蛋白酶2(MMP2)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的基质金属蛋白酶2(MMP2)呈正相关。用化学发光仪测定相对光单位（RLU），计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA100Ra01 | 基质金属蛋白酶2(MMP2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA100Ra01 | 基质金属蛋白酶2(MMP2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA100Ra02 | 基质金属蛋白酶2(MMP2)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA100Ra21 | 基质金属蛋白酶2(MMP2)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA100Ra | 基质金属蛋白酶2(MMP2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA100Ra | 基质金属蛋白酶2(MMP2)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA100Ra | 基质金属蛋白酶2(MMP2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Moleculer Vision | Bone morphogenetic protein-2: a potential regulator in scleral remodeling[PubMed: PMC2605409] |
| Neuroimmunomodulation | Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus[Pubmed: source] |
| Zahedan Journal of Research in Medical Sciences | The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary Men[Zjrms: Source] |
| Experimental Biology and Medicine | Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells[Rsmjournals: Source] |
| Experimental & Translational Stroke Medicine | Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke[PubMed: 21679435] |
| PLoS One | Downregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 [PubMed: PMC3797697] |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source] |
| Clinical and Investigative Medicine | The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells[Pubmed: 23544611] |
| Physiol. Res. | The unwounded skin remodeling in animal models of diabetes types 1 and 2.[Pubmed: 24020818] |
| Acta Histochemica | RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cells[Pubmed: 24681113] |
| Peritoneal Dialysis | Can effluent matrix metalloproteinase 2 and plasminogen activator inhibitor 1 be used as biomarkers of peritoneal membrane alterations in peritoneal dialysis patients?[Pubmed: 23994841] |
| Polski Przeglad Chirurgiczny | Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course[Pubmed: 23585206] |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke[Pubmed:24671655] |
| Int Immunopharmacol.? | Cadherin-11 involves in synovitis and increases the migratory and invasive capacity of fibroblast-like synoviocytes of osteoarthritis[Pubmed:25824718] |
| Journal of Perinatology | Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion[Pubmed:25078863] |
| Biomedical Materials | In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study[Pubmed:25634132] |
| Stem Cells International | Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro[Pubmed:25767513] |
| Physiology and Pharmacology | A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary Men[Phypha:Source] |
| Mediators of Inflammation | Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse[Journals: Mi] |
| Journal of Biomaterials and Tissue Engineering | MicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1[Content: Asp] |
| Biomedical Materials | In vivo[PubMed: 25634132] |
| Int J Clin Exp Pathol | Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathways[PubMed: 26191138] |
| Hattori and Ishihara Stem Cell Research & Therapy | Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cells[PubMed: 25884474] |
| Biosens Bioelectron | Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V3+[PubMed: 26433066] |
| Veterinary Parasitology | Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis[PubMed: 26412140] |
| Canadian Journal of Physiology and Pharmacology | Resveratrol reduces matrix metalloproteinases and alleviates intrahepatic cholestasis of pregnancy in rats[Doi: Abs] |
| PLASTIC AND AESTHETIC RESEARCH | A comparative study to evaluate the effect of limited access dressing on diabetic ulcers[Article: 2347-9264] |
| Int J Mol Sci | Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPARγ[PubMed: 26703570] |
| Basic Research | CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthma[Journal:27699] |
| International Journal of Oncology | 1, 25-Dihydroxyvitamin D3 alleviates salivary adenoid cystic carcinoma progression by suppressing GPX1 expression through the NF-κB pathway[Pubmed:26782341] |
| International Journal of Pharmacology | Research Article Effects of DNMT and HDAC Inhibitors (RG108 and Trichostatin A) on NGF-induced Neurite Outgrowth and Cellular Migration[profile:57162df608aebf0697f1843d] |
| Journal of Stroke and Cerebrovascular Diseases | Epigallocatechin Gallate Extends the Therapeutic Window of Recombinant Tissue Plasminogen Activator Treatment in Ischemic Rats[Pubmed:26851971] |
| VirusDisease | Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection[Pubmed:26925446] |
| Cell Physiol Biochem. | MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1[Pubmed:27160777] |
| Int Wound J. | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases.[Pubmed:26991748] |
| Biosensors and Bioelectronics | Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V2+ conjugates[science:S0956566315304474] |
| world journal of surgery | Metalloproteinases and Their Inhibitors in Patients with Inguinal Hernia.[pubmed:28050662] |
| disease markers | Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases.[pubmed:28348451] |
| Balkan Medical Journal | Anti-inflammatory and Anti-apoptotic Effect of Valproic Acid and Doxycycline Independent from MMP Inhibition in Early Radiation Damage[pubmed:27761275] |
| Microvascular Research | Pleiotropic effects of survivin in vascular endothelial cells.[pubmed:27373518] |
| BMC Veterinary Research | Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy incombination with environmental dust reduction in equine recurrent airway obstruction.[pubmed:27938355] |
| Cellular Physiology and Biochemistry | MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1.[pubmed:27160777] |
| Journal of Biomaterials and Tissue Engineering | MicroRNA-340 Targets NF-κB1 to Inhibit Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma Cell Line[publication:306067745] |
| World Journal of Plastic Surgery | Superficial Burn Wound Healing with Intermittent Negative Pressure Wound Therapy UnderLimited Access and Conventional Dressings.[pubmed:27853690] |
| Journal of Biomaterials and Tissue Engineering | Zoledronic Acid Inhibits TGF-β1-Induced the Proliferation, Migration and EMT Through Smad Pathway in Human Gingival Fibroblasts[art00008] |
| Human cell | Liver X receptor α (LXRα) promoted invasion and EMT of gastric cancer cells by regulation of NF-κB activity[pubmed:28091828] |
| Human Cell | MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1[pubmed:27743351] |
| Experimental Parasitology | Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis[pubmed:28821422] |
| Human Cell | MicroRNA-30a functions as tumor suppressor and inhibits the proliferation and invasion of prostate cancer cells by down-regulation of SIX1[pubmed:28573504] |
| Cellular Physiology and Biochemistry | The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells[pubmed:29224019] |
| International Wound Journal | From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases[pubmed:26991748] |
| Fundamental & Clinical Pharmacology | Epigallocatechin-3-gallate ameliorates intrahepatic cholestasis of pregnancy by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9[DOI:10.1111/fcp.12294] |
| PLoS One | Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis[pubmed:28665955] |
| Nanotoxicology | Cellular proliferation and differentiation induced by single-layer molybdenum disulfide and mediation mechanisms of proteins via the Akt-mTOR-p70S6K signaling pathway[10.1080/17435390.2017.1357213] |
| Archives of Medical Science | CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma.[pubmed:28507585] |
| Molecular Medicine Reports | Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritis[pubmed:28901438] |
| European Journal of Medicinal Chemistry | Syntenin-targeted peptide blocker inhibits progression of cancer cells[Pubmed:29857221] |
| Journal of Neurochemistry | Liraglutide ameliorated peripheral neuropathy in diabetic rats: Involvement of oxidative stress, inflammation and extracellular matrix remodeling[Pubmed:29572844] |
| Journal of Cellular Biochemistry | Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia …[10.1002:jcb.26849] |
| Inflammation | Extracellular Matrix Remodeling and Modulation of Inflammation and Oxidative Stress by Sulforaphane in Experimental Diabetic Peripheral Neuropathy[Pubmed:29704151] |
| OncoTargets and Therapy | sirT4 acts as a tumor suppressor in gastric cancer by inhibiting cell proliferation, migration, and invasion[Pubmed:30022839] |
| Biomed Research International | CD30 Is Highly Expressed in Chronic Obstructive Pulmonary Disease and Induces the Pulmonary Vascular Remodeling[Pubmed:29984229] |
| APMIS | Hepatoprotectivity of Panduratin A against liver damage: demonstration with a rat model of cirrhosis induced by thioacetamide[Pubmed:30058214] |
| Molecular Medicine Reports | Effect of a synthetic inhibitor of urokinase plasminogen activator on the migration and invasion of human cervical cancer cells in vitro[Pubmed:29328476] |
| Oncology Letters | miRNA‑199a‑5p suppresses proliferation and invasion by directly targeting NF‑κB1 in human ovarian cancer cells[10.3892:ol.2018.9170] |
| Taiwanese Journal of Obstetrics & Gynecology | HMGB1-RAGE signaling pathway in pPROM[Pubmed:29673663] |
| Open Medicine | MMP-2 and TIMP-2 in patients with heart failure and chronic kidney disease[Pubmed:29915813] |
| Pediatric Research | Anti-thymic stromal lymphopoietin antibody suppresses airway remodeling in asthma through reduction of MMP and CTGF.[Pubmed: 30464333] |
| Advanced Healthcare Materials | Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts[Pubmed: 30838809] |
| Cellular & Molecular Biology Letters | MiR-613 inhibits proliferation and invasion and induces apoptosis of rheumatoid arthritis synovial fibroblasts by direct down-regulation of DKK1[] |
| Mediators of Inflammation | Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma[] |
| Journal of endovascular therapy: an official journal of the International Society of Endovascular Specialists | Correlation of Baseline Plasma and Inguinal Connective Tissue Metalloproteinases and Their Inhibitors With Late High-Pressure Endoleak After Endovascular …[Pubmed: 31464166] |
| Oncology Research | MicroRNA-6884-5p regulates the proliferation, invasion and EMT of gastric cancer cells by directly targeting S100A16[Pubmed: 31796150] |
| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis[Pubmed: 31980722] |
| Acta Biomaterialia | Stiffness of the Aligned Fibers Affects Structural and Functional Integrity of the Oriented Endothelial Cells[Pubmed: 32205213] |
| JOURNAL OF THROMBOSIS AND THROMBOLYSIS | Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertension[Pubmed: 33175289] |
| JOURNAL OF ETHNOPHARMACOLOGY | Danlou tablet inhibits the inflammatory reaction of high-fat diet-induced atherosclerosis in ApoE knockout mice with myocardial ischemia via the NF-κB signaling …[Pubmed: 32745509] |
| heliyon | Matrix levels of metalloproteinase-2 (MMP-2) and toxicity evaluation of carbonate apatite-based endodontic sealer in rat subcutaneous implantation[Pubmed: 32671254] |
| J Cell Physiol | A novel role of VEPH1 in regulating AoSMC phenotypic switching[Pubmed: 32342520] |
| Advances in Animal and Veterinary Sciences | Clinicopathological studies and new markers for trypanosoma evansi in experimen-tally infected rats[] |
| Alteration on MMP-2 Levels in Women Diabetes Patients in Basrah[] | |
| Veterinary Sciences: Research and Reviews | Investigation of matrix metalloproteinase-2 and matrix metalloproteinase-9 activity in some inflammatory conditions in Barki sheep[] |
| MEDIATORS OF INFLAMMATION | MMP-9 Concentration in Peritoneal Fluid Is a Valuable Biomarker Associated with Endotoxemia in Equine Colic[33488296] |
| Oncol Lett | GATA3 is downregulated in HCC and accelerates HCC aggressiveness by transcriptionally inhibiting slug expression[33613720] |
| Journal of Pregnancy | Matrix Metalloproteinases MMP-2 and MMP-9 Occupy a New Role in Severe Preeclampsia[33381317] |
| Immunopharmacol Immunotoxicol | Tofacitinib enhances remyelination and improves myelin integrity in cuprizone-induced mice[34618622] |
| Exp Cell Res | Netrin-like domain of sFRP4, a Wnt antagonist inhibits stemness, metastatic and invasive properties by specifically blocking MMP-2 in cancer stem cells from human …[34762897] |
| Sci Rep | Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells[34837026] |
| Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and EMT Process in Gastric Cancer[34650982] | |
| Medicina (Kaunas) | Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study[Pubmed:35743980] |
| Journal of Pharmaceutical Research International | Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid …[] |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …[] | |
| Turkish Journal of Biochemistry-Turk Biyokimya Dergisi | Downregulation of MMP-2 and MMP-9 genes in obesity patients and their relation with obesity-related phenotypes[] |