睾酮(Testo)检测试剂盒(化学发光免疫分析法)
CLIA Kit for Testosterone (Testo)
T; Testesteron; Testeronun
- 编号CCA458Ge
- 物种Pan-species (General，通用)相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围117.2-30,000pg/mL
- 灵敏度最小可检测剂量小于等于40.5pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载英文说明书 中文说明书
- 规格48T96T96T*596T*1096T*100
- 价格¥ 3427¥ 4896¥ 22032¥ 41616¥ 342720
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
特异性
本试剂盒用于检测睾酮(Testo)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的睾酮(Testo)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 94-102 | 97 |
| EDTA plasma(n=5) | 78-101 | 85 |
| heparin plasma(n=5) | 96-105 | 101 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的睾酮(Testo)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中睾酮(Testo)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-101% | 90-104% | 88-95% | 84-94% |
| EDTA plasma(n=5) | 81-101% | 84-104% | 85-99% | 91-105% |
| heparin plasma(n=5) | 84-97% | 78-102% | 78-91% | 89-98% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加底物100µL，37°C孵育10分钟；
7. 读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将睾酮(Testo)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原（标准品或样本），待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，再次彻底洗涤后加入底物。加入底物后会产生辉光型光信号。发射光强度和样品中的睾酮(Testo)呈负相关。用化学发光仪测定相对光单位（RLU），计算样品浓度。
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CPA458Ge11 | 睾酮(Testo)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA458Ge21 | 睾酮(Testo)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA458Ge01 | 睾酮(Testo)多克隆抗体 | ELISA |
| PAA458Ge02 | 睾酮(Testo)多克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA458Ge71 | 睾酮(Testo)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA458Ge27 | 睾酮(Testo)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA458Ge22 | 睾酮(Testo)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA458Ge23 | 睾酮(Testo)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA458Ge24 | 睾酮(Testo)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA458Ge25 | 睾酮(Testo)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA458Ge21 | 睾酮(Testo)单克隆抗体 | ELISA |
| MEA458Ge | 睾酮(Testo)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA458Ge | 睾酮(Testo)检测试剂盒(酶联免疫吸附试验法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA458Ge | 睾酮(Testo)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA458Ge | 睾酮(Testo)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA458Ge | 睾酮(Testo)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA458Ge | 睾酮(Testo)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA458Ge11 | 睾酮(Testo)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
| KSA458Ge12 | 睾酮(Testo)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| The Tohoku Journal of Experimental Medicine | Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-β in Orchiectomized Mature Rats[Jstage: source] |
| Plos one | Insulin Receptor Substrate 2 Is Required for Testicular Development[PubMed: PMC3669358] |
| International Journal of Molecular and Cellular Medicine | Central effects of camphor on GnRH and sexual hormones in male rat[Pubmed: 24551777] |
| Fertility and Sterility | Growth differential factor-9 inhibits testosterone production in mouse theca interstitial cells [Pubmed: 23954356] |
| Urologia internationalis | Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats[Pubmed: 24051982] |
| International Braz J Urol | The effect of sertraline, paroxetine, fluoxetine and escitalopram on testicular tissue and oxidative stress parameters in rats.[Pubmed: 24642156] |
| Journal of Materials Chemistry B | Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering[Rsc: Source] |
| Biochemical and Biophysical Research Communications | Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis[ScienceDirect: S0006291X14001843] |
| PLoS One. | Diets rich in saturated and polyunsaturated fatty acids induce morphological alterations in the rat ventral prostate[Pubmed:Pmc4100933] |
| Reproductive Toxicology | Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring[Pubmed:24126190] |
| Physiology & Behavior | The impact of maternal consumption of cafeteria diet on reproductive function in the offspring[Pubmed:24631302] |
| Int Braz J Urol. | The effect of Sertraline, Paroxetine, Fluoxetine and Escitalopram on testicular tissue and oxidative stress parameters in rats[Pubmed:24642156] |
| Environmental Research | Perfluorooctane sulfonate effects on the reproductive axis in adult male rats[Pubmed:25171141] |
| Acta Cir Bras. | Lard and/or canola oil-rich diets induce penile morphological alterations in a rat model.[Pubmed:25185055] |
| Genet Mol Biol. | Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility[Pubmed:25505846] |
| Turkish Journal of Urology | Apoptosis in testicular tissue of rats after vasectomy: evaluation of eNOS, iNOS immunoreactivities and the effects of ozone therapy[Turkishjournalofurology:Source] |
| Int J Clin Exp Med | Effects of sericin on the testicular growth hormone/insulin-like growth factor-1 axis in a rat model of type 2 diabetes[PubMed: 26379831] |
| Int Braz J Urol | Metabolic and Testicular Effects of the Long-Term Administration of Different High-Fat Diets in Adult Rats[PubMed: 26200553] |
| Advances in Environmental Biology | Plasma Testosterone and Testis Histological Features of Mice Treated with Averrhoa Bilimbi, Cosmos Caudatus and Pereskia Bleo Ethanolic Extract[Aensiweb: Aeb] |
| international journal of environmental research and public health | Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy[PubMed: 25996886] |
| Environ Toxicol | Effect on the H20 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period[PubMed: 25782374] |
| J Endocrinol | Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction[PubMed: 25770118] |
| Acta Pharmaceutica Sinica B | Preparation and evaluation of a phospholipid-based injectable gel for the long term delivery of leuprolide acetaterrh[science:S2211383516301812] |
| Plos One | Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota[Pubmed:27093642] |
| Theriogenology | Expression of androgen-producing enzyme genes and testosterone concentration in AnCavia (Guinea pig )s and Nellore heifers with high and low ovarian follicle count[Pubmed:26948295] |
| PPAR Research | Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARα[Pubmed:27413362] |
| TURKISH JOURNAL OF MEDICAL SCIENCES | THE EFFECT OF KISSPEPTIN ON SPERMATOGENESIS AND APOPTOSIS IN RATS[fileID:693211] |
| Int J Mol Sci. | Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice[Pubmed:26771609] |
| Scientific Reports | Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus[pubmed:27461373] |
| Journal of Applied Life Sciences International | Maternal Sleep Deprivation Alters Reproductive Capability of Male Offspring in Wistar Rats[Akindele922016JALSI29782.pdf] |
| Integrative Zoology | Recovery of fertility in quinestrol-treated or diethylstilbestrol-treated mice: Implications for rodent management.[pubmed:27611741] |
| 33 | Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.[pubmed:28336389] |
| environmental pollution | Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum.[pubmed:27838061] |
| PPAR Research | Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction by Upregulating PPARα[pubmed:27413362] |
| Andrologia | Effects of treatment with Hypoxis hemerocallidea extract on sexual behaviour and reproductiveparameters in male rats.[pubmed:28000943] |
| Turkish journal of medical sciences | The effect of kisspeptin on spermatogenesis and apoptosis in rats.[pubmed:28263511] |
| Kafkas Universitesi Veteriner Fakultesi Dergisi | The Angiogenic Effect of Testosterone Supplementation on Brain of Aged Mice.[13006045] |
| Effect of dietary fish oil on mouse testosterone level and the distribution of eicosapentaenoic acid-containing phosphatidylcholine in testicular interstitium[S2405580816300991] | |
| Reproductive Sciences | Abnormality of Klotho Signaling Is Involved in Polycystic Ovary Syndrome[pubmed:28673204] |
| Biomedicine & Pharmacotherapy | Toxic effects of Tripterygium wilfordii Hook F on the reproductive system of adolescent male rats[pubmed:28938525] |
| Molecular and Cellular Endocrinology | Testosterone deficiency prevents left ventricular contractility dysfunction after myocardial infarction [pubmed:28606867] |
| Ankara Üniv Vet Fak Derg | The effect of testosterone supplementation on morphometry and VEGF expression level of thoracic aorta in aged mice[DOI: 10.1501/Vetfak_0000002791] |
| Acta histochemica | Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-)[pubmed:28506466] |
| DARU Journal of Pharmaceutical Sciences | Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway[pubmed:29020999] |
| Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population[Pubmed:29525625] |
| Aquatic Toxicology | Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrations[Pubmed:29775925] |
| Molecular Reproduction and Development | deficiency leads to testicular dysfunction and altered expression of genes involved in cell communication, chromatin reorganization, spermatogenesis, and …[Pubmed:29411926] |
| Journal of Ovarian Research | DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway[Pubmed:29321035] |
| Medical Science Monitor | Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) …[Pubmed:29627845] |
| Oncology Letters | Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes …[10.3892:ol.2018.9165] |
| Biomedicine & Pharmacotherapy | Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons[Doi: 10.1016/j.biopha.2018.08.135] |
| Asian Journal of Andrology | Resveratrol attenuates metabolic, sperm, and testicular changes in adult Wistar rats fed a diet rich in lipids and simple carbohydrates[Pubmed: 30198494] |
| 第三军医大学学报 | 基因敲除损伤小鼠睾丸睾酮产生与生精功能[] |
| Stem Cells Translational Medicine | Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats[Pubmed: 30569668] |
| Brazilian Journal of Pharmaceutical Science | Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients[] |
| Journal of Endocrinology | Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome[Pubmed: 29535146] |
| Journal of Cellular Physiology | Brain‐derived neurotrophic factor: A steroidogenic regulator of Leydig cells[Pubmed: 30628054] |
| Experimental and Therapeutic Medicine | Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary[] |
| Evidence-Based Complementary and Alternative Medicine | iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine[] |
| International Journal of Reproductive Biomedicine | The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study.[] |
| Kafkas Universitesi Veteriner Fakultesi Dergisi | The Effect of Testosterone Supplementation on Capillar Morphometry and VEGF Expression Level of the Heart in Aged Mice[] |
| Journal of Cellular and Molecular Medicine | Cyclophilin D participates in the inhibitory effect of high‐fat diet on the expression of steroidogenic acute regulatory protein[Pubmed: 31373170] |
| Journal of neuroscience research | Revisiting the relationships of 2D:4D with androgen receptor (AR) gene and current testosterone levels: Replication study and meta‐analyses[Pubmed: 31359506] |
| Andrologia | Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male rats[Pubmed: 31353623] |
| Chemosphere | Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling[Pubmed: 31809938] |
| science of the total environment | Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cells[Pubmed: 32334352] |
| JOURNAL OF MOLECULAR NEUROSCIENCE | Serum Progesterone and Testosterone Levels in Schizophrenia Patients at Different Stages of Treatment[Pubmed: 33159671] |
| BIOLOGICAL RESEARCH | Novel mechanisms underlying anti-polycystic ovary like syndrome effects of electroacupuncture in rats: suppressing SREBP1 to mitigate insulin resistance …[Pubmed: 33109277] |
| J Food Biochem | Novel antihypertensive action of rutin is mediated via inhibition of angiotensin converting enzyme/mineralocorticoid receptor/angiotensin 2 type 1 receptor (ATR1) …[Pubmed: 33089540] |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Parental exposure to polystyrene microplastics at environmentally relevant concentrations has negligible transgenerational effects on zebrafish (Danio rerio)[Pubmed: 33011509] |
| ARCHIVES OF GYNECOLOGY AND OBSTETRICS | MiRNAs expression profiling of rat ovaries displaying PCOS with insulin resistance[Pubmed: 32757043] |
| Mol Med | Electroacupuncture alleviates polycystic ovary syndrome-like symptoms through improving insulin resistance, mitochondrial dysfunction, and endoplasmic …[Pubmed: 32698821] |
| Antioxidants (Basel) | Composition of Royal Jelly (RJ) and Its Anti-Androgenic Effect on Reproductive Parameters in a Polycystic Ovarian Syndrome (PCOS) Animal Model[Pubmed: 32517356] |
| Andrologia | Maternal CBZ exposure impairs testicular development, spermatogenesis and sperm parameters in male offspring at puberty[Pubmed: 32478927] |
| chemosphere | N, N-Dimethylacetamide, an FDA approved excipient, acts post-meiotically to impair spermatogenesis and cause infertility in rats[Pubmed: 32447106] |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress[Pubmed: 32685488] |
| Iranian Journal of Basic Medical Sciences | Therapeutic effects of organic zinc on reproductive hormones, insulin resistance and mTOR expression as a novel component in a rat model of PCOS[] |
| IVERMECTINA IMPEDE DANOS TESTICULARES EM RATOS[] | |
| The effects of gold nanoparticles on Leydig cells and male reproductive function in mice[] | |
| The Effect of Acupuncture on MiRNA Expression Profiles in the Ovaries of Rats with PCOS and Insulin Resistance[] | |
| JOURNAL OF ETHNOPHARMACOLOGY | BaZiBuShen alleviates altered testicular morphology and spermatogenesis and modulates Sirt6/P53 and Sirt6/NF-¦ÊB pathways in aging mice induced by D-galactose[33508368] |
| Toxicol Appl Pharmacol | Exposure to the bromodomain inhibitor N-methyl pyrrolidone blocks spermatogenesis in a hormonal and non-hormonal fashion[33965371] |
| African Journal of Traditional, Complementary and Alternative Medicines | A comparative study on the aphrodisiac activity of food plants< i> Mondia whitei, Chenopodium album, Ccucurbita pe...[] |
| Bioengineered | MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 1[34637688] |
| J Virol | Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice[34730391] |
| Eur J Pharmacol | Yohimbine hydrochloride inhibits benign prostatic hyperplasia by downregulating steroid 5α-reductase type 2[34265299] |
| Theriogenology | Superior effect of broccoli methanolic extract on control of oxidative damage of sperm cryopreservation and reproductive performance in rats: A comparison with …[Pubmed:35063921] |
| Reprod Sci | Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in Mice[Pubmed:35257354] |