25-羟基维生素D3(HVD3)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for 25-Hydroxyvitamin D3 (HVD3)
25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25(OH)D
- 编号KSA915Ge11
- 物种Pan-species (General，通用)相同的名称，不同的物种。
- 试剂内容包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma and other biological fluids.
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围2.47-200ng/mL
- 可适灵敏度0.91ng/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 8597¥ 14328¥ 25790¥ 50148¥ 85968
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对25-羟基维生素D3(HVD3)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Pan-species (General，通用) | 应用(仅供研究使用，不用于临床诊断！) |
| CPA915Ge11 | 25-羟基维生素D3(HVD3)牛血清白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CPA915Ge21 | 25-羟基维生素D3(HVD3)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| PAA915Ge01 | 25-羟基维生素D3(HVD3)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA915Ge71 | 25-羟基维生素D3(HVD3)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA915Ge22 | 25-羟基维生素D3(HVD3)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA915Ge21 | 25-羟基维生素D3(HVD3)单克隆抗体 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEA915Ge | 25-羟基维生素D3(HVD3)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA915Ge | 25-羟基维生素D3(HVD3)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA915Ge11 | 25-羟基维生素D3(HVD3)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Allergy & Clinical Immunology | Decreased serum LL-37 and vitamin D3 levels in atopic dermatitis: relationship between IL-31 and oncostatin M[Wiley: source] |
| Tianjin Med J | 成人哮喘血清维生素D 水平与肺功能相关因素分析[Jwk_Tjyy: Cn] |
| Journal of Functional Foods | Comparative effects of brown and golden flaxseeds on body composition, inflammation and bone remodelling biomarkers in perimenopausal overweight women[S1756464617301561] |
| J Nutr Biochem. | Chronic maternal calcium and 25-hydroxyvitamin D deficiency in Wistar rats programs abnormal hepatic gene expression leading to hepatic steatosis in female offspring.[pubmed:28219837] |
| in vivo | Effect of Calcitriol on FGF23 Level in Healthy Adults and its Dependence on Phosphate Level[pubmed:28064234] |
| Asia pacific allergy | Serum 25-hydroxyvitamin D inversely associated with blood eosinophils in patients with persistent allergic rhinitis.[pubmed:29094019] |
| Biomed Research International | Effects of Vitamin D Restricted Diet Administered during Perinatal and Postnatal Periods on the Penis of Wistar Rats[Pubmed:29850540] |
| Clin Implant Dent Relat Res. | Evaluation of FGF‐23 and 25(OH)D3 levels in peri‐implant sulcus fluid in peri‐implant health and diseases[Pubmed: 31407857] |
| JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY | Circulating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in …[Pubmed: 33065276] |
| The Journal of Obstetrics and Gynecology of India | The Effects of Follicular Fluid 25 (OH) D Concentration on Intrafollicular Estradiol Level, Oocyte Quality, and Fertilization Rate in Women Who Underwent IVF Program[] |