武汉云克隆科技股份有限公司

聚集蛋白聚糖(AGC)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Aggrecan (AGC)

ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1

  • 聚集蛋白聚糖(AGC)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 聚集蛋白聚糖(AGC)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对聚集蛋白聚糖(AGC)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

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编号适用物种：Homo sapiens (Human，人)应用(仅供研究使用，不用于临床诊断！)
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参考文献

杂志参考文献
Experimental Biology and Medicine Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells[Rsmjournals: Source]
Age( dordr).In vitro tenocyte metabolism in aging and oestrogen deficiency[Springer: Source]
PLoS ONEA Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches[Plosone: Source]
Stem Cell Research & TherapyProtein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly.[Pubmed: 24739658]
Materials Science and Engineering: CCo-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of composition[ScienceDirect: S0928493113006656]
PLoS One.Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media[Pubmed:Pmc4130592]
Connective Tissue ResearchMetabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes[Pubmed:25333747]
Macromolecular Bioscience Highly Porous Gelatin Reinforced 4D Scaffolds for Articular Cartilage Regeneration[PubMed: 25787871]
Osteoarthritis and CartilageParathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized Cavia (Guinea pig )inea pigs[Pubmed:26802547]
Cell ProliferationEffects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes[Pubmed:27090063]
bmc musculoskeletal disordersStrontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis.[pubmed:28187731]
Journal of Clinical MedicineAggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors[Pubmed: 32392807]
Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs[]
researchsquareAggrecan-a new biomarker for acute type A aortic dissection[33990642]