α-黑素细胞刺激素(aMSH)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Alpha-Melanocyte Stimulating Hormone (aMSH)
α-MSH; Intermedins; Alpha-Melanotropin, Alpha-Melanocortin; Alpha-Intermedin
- 编号KSA239Mu11
- 物种Mus musculus (Mouse，小鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、生物素标记的竞争物、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理竞争抑制酶联免疫吸附试验法测抗原
- 可检测范围123.5-10,000pg/mL
- 可适灵敏度43.9pg/mL
- 应用Main materials for "Do It (ELISA Kit) Yourself".
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 6869¥ 11448¥ 20606¥ 40068¥ 68688
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产品包装(模拟)
产品包装(模拟)
通过ISO 9001、ISO 13485质量体系认证
特异性
试剂盒中的抗体对α-黑素细胞刺激素(aMSH)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| CEA239Mu | α-黑素细胞刺激素(aMSH)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA239Mu | α-黑素细胞刺激素(aMSH)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA239Mu11 | α-黑素细胞刺激素(aMSH)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| J Biochem. | Generation of a human bone marrow-derived mesenchymal stem cell line expressing and secreting high levels of bioactive α-melanocyte-stimulating hormone.[Pubmed: 23341471] |
| Pigment Cell Melanoma Res | Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit regulated by light[PubMed: 26582755] |
| The Journal of Biological Chemistry | Intermedin Restores Hyperhomocysteinemia-induced Macrophage Polarization and Improves Insulin Resistance in Mice[Pubmed:27080257] |
| Pigment Cell & Melanoma Research | Pharmacological induction of skin pigmentation unveils the neuroendocrine circuit reCavia (Guinea pig )lated by light[Pubmed:26582755] |
| Nature Communications | Endocrine disruptors induce perturbations in endoplasmic reticulum and mitochondria of human pluripotent stem cell derivatives.[pubmed:28794470] |
| Cell Stem Cell | Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses[Pubmed:29681516] |
| European Neuropsychopharmacology | Epigenetic alterations of the POMC promoter in tobacco dependence[Pubmed:29871818] |
| Neuropeptides | Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factor[Pubmed:29685637] |
| Neuropsychobiology | Alcohol Withdrawal and Proopiomelanocortin Neuropeptides in an Animal Model of Alcohol Dependence[Pubmed: 31117084] |
| INTERNATIONAL IMMUNOPHARMACOLOGY | Intermedin alleviates the inflammatory response and stabilizes the endothelial barrier in LPS-induced ARDS through the PI3K/Akt/eNOS signaling pathway[Pubmed: 32892076] |
| J Endocrinol Invest | Serum alpha-melanocyte-stimulating hormone (a-MSH), brain-derived neurotrophic factor (BDNF), and agouti-related protein (AGRP) levels in children with Prader …[Pubmed:35098494] |