雄烯二酮(ASD)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Androstenedione (ASD)

4-Androstenedione; 4-Androstene-3,17-Dione

  • 雄烯二酮(ASD)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • 雄烯二酮(ASD)检测试剂盒DIY材料(酶联免疫吸附试验法)产品包装(模拟)
  • Certificate通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对雄烯二酮(ASD)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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编号适用物种:Pan-species (General,通用)应用(仅供研究使用,不用于临床诊断!)
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参考文献

杂志参考文献
Plos OneAssociation between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota[Pubmed:27093642]
Scientific ReportsMolecular characterization of insulin resistance and glycolytic metabolism in the rat uterus[pubmed:27461373]
33Metformin Ameliorates Uterine Defects in a Rat Model of Polycystic Ovary Syndrome.[pubmed:28336389]
Journal of EndocrinologyUterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome[Pubmed: 29535146]
International Journal of Biological SciencesMelatonin Stimulates STAR Expression and Progesterone Production via Activation of the PI3K/AKT Pathway in Bovine Theca Cells[]
Molecular reproduction and developmentEvidence that downregulation of Wilms' tumor 1 (WT1) is involved in cortical stromal cell differentiation into theca cells in adult bovine ovaries[Pubmed: 31490589]
DOMESTIC ANIMAL ENDOCRINOLOGYWilms' tumor (WT1)(+/-KTS) variants decreases the progesterone secretion of bovine ovarian theca cells[Pubmed: 32739762]
ANIMAL REPRODUCTION SCIENCEA study on steroidogenic elaborations of stroma and their regulation in response to ovarian hormones in goats[33845412]
Reprod Domest AnimExtracellular Vesicles of Bovine Small Follicular Fluid Promote Ovarian Cortical Stromal Cell Proliferation and Steroidogenesis[34402549]
Curr OncolPlasma Aromatase Activity Index, Gonadotropins and Estrone Are Associated with Frailty Syndrome in Post-Menopausal Women with Breast Cancer[Pubmed:35323344]