前梯度蛋白2(AGR2)检测试剂盒DIY材料(酶联免疫吸附试验法)
ELISA Kit DIY Materials for Anterior Gradient 2 (AGR2)
GOB4; HAG2; XAG2; AG2; PDIA17; HPC8; Anterior Gradient Protein 2; Protein Disulfide Isomerase Family A,Member 17; Secreted cement gland protein XAG-2 homolog
- 编号KSC285Ra01
- 物种Rattus norvegicus (Rat，大鼠)相同的名称，不同的物种。
- 试剂内容包被抗体、检测抗体、标准品、HRP标记的链霉亲和素、TMB底物、96孔板
- 可检测样本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可适用原理双抗夹心酶联免疫吸附试验法测抗原
- 可检测范围1.56-100ng/mL
- 可适灵敏度0.62ng/mL
- 应用自己动手制作ELISA试剂盒的主要材料
- 下载英文说明书 中文说明书
- 规格96T*596T*1096T*2096T*5096T*100
- 价格¥ 7423¥ 12372¥ 22270¥ 43302¥ 74232
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特异性
试剂盒中的抗体对前梯度蛋白2(AGR2)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。
用法
1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。
储存
抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。
配套试剂
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC285Ra01 | 前梯度蛋白2(AGR2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC285Ra01 | 前梯度蛋白2(AGR2)多克隆抗体 | IHC |
| LAC285Ra71 | 前梯度蛋白2(AGR2)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAC285Ra22 | 前梯度蛋白2(AGR2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC285Ra23 | 前梯度蛋白2(AGR2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC285Ra26 | 前梯度蛋白2(AGR2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC285Ra21 | 前梯度蛋白2(AGR2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC285Ra24 | 前梯度蛋白2(AGR2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC285Ra27 | 前梯度蛋白2(AGR2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAC285Ra25 | 前梯度蛋白2(AGR2)单克隆抗体 | WB; IHC; ICC; IP. |
| KSC285Ra01 | 前梯度蛋白2(AGR2)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Cancer Biomarkers | Serum AGR2 as an early diagnostic and postoperative prognostic biomarker of human lung adenocarcinoma[MetaPress: n7137r6271u54118] |
| Molecular & Cellular Proteomics | Integrated Proteomic Profiling of Cell Line Conditioned Media and Pancreatic Juice for the Identification of Pancreatic Cancer Biomarkers[PubMed: PMC3205865] |
| Prostate. | Anterior gradient 2 (AGR2): blood-based biomarker elevated in metastatic prostate cancer associated with the neuroendocrine phenotype[Pubmed: 22911164] |
| BMC Cancer. | Validation of four candidate pancreatic cancer serological biomarkers that improve the performance of CA19.9[Pubmed: 24007603] |
| Clinical Cancer Research | Validation of Biomarkers That Complement CA19.9 in Detecting Early Pancreatic Cancer[Pubmed:25239611] |
| PLoS One | AGR3 in Breast Cancer: Prognostic Impact and Suitable Serum-Based Biomarker for Early Cancer Detection[PubMed: 25875093] |
| Clinical Neurology and Neurosurgery | Serum AGR2 as a useful biomarker for pituitary adenomas[S0303846717300045] |
| Oncotarget | Identification, characterization and application of a new peptide against anterior gradient homolog 2 (AGR2)[Pubmed:29937991] |
| Int J Oncol | Anterior gradient protein 2 is a marker of tumor aggressiveness in breast cancer and favors chemotherapy‑induced senescence escape[34913074] |
| International Journal of Cancer | Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancer[Pubmed:35689436] |